【摘要】 目的　探讨肿瘤可溶性抗原免疫原性,为肿瘤疫苗的研制和肿瘤免疫治疗提供实验依据。方法　提取卵巢癌细胞的可溶性成分作为肿瘤可溶性抗原(tumorsolubleantigen,TSA),单独用TSA或联合IL-2,CD-3单抗诱导外周血单个核细胞(PBMC),检测PBMC活化、增殖、分泌细胞因子以及活化的细胞对肿瘤的杀伤特性。结果　TSA能刺激正常人PBMC增殖和分泌细胞因子(TNF,IFN)。用TSA联合IL-2和抗CD3单抗诱导正常人PBMC产生杀瘤性效应细胞,对TSA来源的肿瘤细胞具有选择性杀伤作用,证实TSA具有免疫原性。肿瘤细胞提取物中细胞多肽是主要活性成分,但细胞膜成分也具有免疫活性。结论　用该实验方法提取TSA具有免疫原性,可用于肿瘤疫苗的研制和肿瘤过继免疫治疗,该实验方法和结果可为检测和评价肿瘤提取物是否具有免疫原性提供参考。更多还原

【Abstract】 Objectives To study the immunoginicity of soluble antigen of ovarian cacinoma for the development of tumor vaccine and tumor immunotherapy.Methods Tumor Soluble Antigen(TSA) was extracted from human ovarian carcinoma cell lines.Peripheral blood mononuclear cells(PBMC)from normal donors were stimulated in vitro by TSA or TSA with anti CD3 monoantibody and IL-2 to develop into Cytotoxic T Lymphocytes(CTL). The activation, proliferation and cytotoxicity of th CTL against different tumor cells were detceted.Results The CTLs can proliferate and produce lymphokines (TNF,IFN).PBMC were induced by TSA and anti CDs monoclonal antibody and cultured in medium with IL 2 to generate Cytotoxic T Lymphocytes (CTL).The cytotoxicity showed selectively against tumor cells that came from TSA, which proved that immunogenicity existed in TSA.Further analysis of immunoginicity of TSA showed that main activity was in polypeptides and of membrance protein. Conclusions There are immunogenicity in TSA for the application in development of tumor vaccine and immunitheraty of tumor. Our experimental methods and results might provide reference for the evaluate of immunigenicity of tumor antigen and experimental base for the development of tumor vaccine and immunotherapy of tumor.更多还原