【摘要】 目的：建立血清抗Sm抗体的ELISA检测方法。方法：应用纯化的Sm抗原，采用棋盘滴定等方法确立ELISA方法检测血清抗Sm抗体的最适实验条件。结果：建立了血清抗Sm抗体的ELISA检测方法。SLE患者血清抗Sm抗体的阳性率为29．2％，正常人和其它自身免疫病患者未查到此抗体。结论：本文建立的血清抗Sm抗体检测方法敏感性达到国外进口试剂盒的水平，且操作简便，重复性好，因此临床很有实用价值。更多还原

【Abstract】 objective: To establish ELISA for anti - Sm antibody in serum. Methods: Using purified SmAg and Chess Board Titraing method, we set up the optimal experimental conditions of FLISA for anti - Sm antibody. Result: The ELISA for anti- Sm antibody in serum from SLE was established. The positive rate of anti - Sm antibody in serum from SLF was 29. 2%. There was no anti - Sm antibody in normal and other autoimmune disease controls. Conclusion: The sensitivity of the ELISA for anti -Sm antibody approched to abroad ELISA Kit. In addition, it is easy and reproducible. so, it is practical clinically.更多还原