【摘要】 目的　观察ｃｙｃｌｉｎ Ｅ 的高表达对乳腺癌细胞ＭＣＦ７ 生长及周期的影响。方法　构建ｃｙｃｌｉｎ ＥｃＤＮＡ真核表达载体并采用ｌｉｐｏｆｅｃｔＡＭＩＮＥ 转染方法将其导入ＭＣＦ７ 细胞，获得稳定表达ｃｙｃｌｉｎ Ｅ的细胞系。通过对细胞生长曲线绘制、３ＨＴｄＲ测定及细胞周期分布等的分析，观察其对细胞生长、增殖的影响。结果　ｃｙｃｌｉｎ Ｅ的高表达可以使细胞的生长速度加快（ 约为对照１－５２ 倍） 及ＤＮＡ 掺入增加（４－２ 倍） ，Ｇ１Ｓ移行加速；ｐＲＢ的磷酸化形式的增多（３ 倍） 。结论　ｃｙｃｌｉｎ Ｅ的高表达可明显影响ＭＣＦ７ 细胞的生长、增殖，并可能通过ｐＲＢ 磷酸化，影响细胞周期Ｇ１Ｓ期移行而实现其对生长的调节。更多还原

【Abstract】 Objective To investigate the effect of cyclin E expression on cells growth and cell cycle changes of human breast cancer MCF 7 cell line.Methods The recombination eukryotic expression plasmid of cyclin E was constructed, and transferred into the MCF 7 cell line with lipofectAMINE.Cell clones were selected with G418.Cells growth properties, 3H TdR and cell cycle analysis were done.Results The cyclin E overexpression could obviously increase the cells growth speed (1 52 fold higher than vector control) and DNA synthesis (1 75 fold) and also increase the cells progression from G1 to S phase.The hyperphosphorylation form of pRB was increased (3 fold).Conclusion High expression of cyclin E could effect the cell growth and cell proliferation probably through regulating the phosphorylation of pRB and G1 S progression.更多还原