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免疫表型分型结合FISH检测自然杀伤细胞淋巴瘤细胞的染色体畸变
The Detection of Chromosome Aberrations in Natural Killer Cell Lymphoma/leuk1emia with a Combination of Immunophenotyping and FISH
【摘要】 目的：检测自然杀伤（ＮＫ）细胞淋巴瘤中含有染色体畸变的肿瘤细胞的免疫表型。方法：采用免疫表型分型结合荧光原位杂交（ＦＩＳＨ）技术，在单细胞水平检测了３例ＮＫ细胞淋巴瘤中含有染色体畸变的肿瘤细胞的免疫表型。结果：在该３例中，克隆性的染色体畸变仅见于ＣＤ５６＋ 细胞，在ＣＤ３＋ 细胞和ＣＤ５＋ 细胞中未发现相应染色体畸变。结论：实验表明，ＮＫ细胞淋巴瘤中，含有染色体畸变的肿瘤细胞局限于ＣＤ５６＋ 、ＣＤ３－ 和ＣＤ５－ 的ＮＫ细胞群，ＮＫ细胞淋巴瘤可能来源于成熟的ＮＫ细胞而非Ｔ淋巴细胞
【Abstract】 Objective:To detect the immunophenotypes of tumor cells with chromosome aberrations in three cases of natural killer (NK) cell lymphoma/leukemia.Methods:A combination of immunophenotyping and FISH.Results:In all three cases,clonal chromosome aberrations were detected only in CD56+ cells,not in CD3+ or CD5+ cells.Conclusion:This study indicates that chromosome aberrations in NK cell lymphoma/leukemia might be restricted to the CD56+,CD3- and CD5- cell populations and that NK cell lymphoma/leukemia may derive from mature NK cells and not from T lymphocytes.
【Key words】 Lymphoma,non Hodgkin’s/etiol； Killer cells,natural； Immunophenotyping； Chromosome aberrations； In situ hybridization,fluorescence； Antigens,CD56/anal；
- 【文献出处】 浙江大学学报(医学版) ,JOURNAL OF ZHEJIANG UNIVERSITY(MEDICINE EDITION) , 编辑部邮箱 ,1999年06期
- 【分类号】R733.1
- 【下载频次】36