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利用γ射线辐照诱发水稻龙特甫B叶色突变

Preliminary study on adding to leaf color marker for rice cytoplasmic male sterile (CMS) line long te fu A by mutation technology

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【作者】 陈善福; 舒庆尧; 吴殿星; 崔海瑞; 夏英武;

【Author】 CHEN Shan fu, SHU Qing yao, WU Dian xing, CUI Hai rui, XIA Ying wu (Institute of Nuclear Agricultural Sciences, Zhejiang University, Hangzhou 310029, China) . Journal of Zhejiang University (Agric. & Life Sci.), 1999,25

【机构】 浙江大学原子核农业科学研究所!浙江杭州310029;

【摘要】 用60Co-γ射线直接辐照龙特甫B干种子,诱发获得了多种类型叶色突变体。M2 代按苗计,白化、黄化和条纹三种叶色突变体的频率依次为0.347% ,0.041% 和0.031% 。对M2 代黄化和条纹两类突变群体的生长发育动态研究发现,其平均叶片数和分蘖数与对照有较大的差异。但有一些黄化和条纹突变体在三叶期后即可转为绿色,其叶片数和分蘖数与对照相近。在用这些叶色突变体与龙特甫A 杂交、回交后的M 4BC1 群体中,分离出正常绿色和带有叶色标记的两类不育株,二者之比接近1∶1。根据苗期和成株期的叶色特征,这些突变系分为 6 种类型。对M 4 突变系的株高、单株穗数、每穗总粒数、每穗实粒数和结实率及相应的 M4BC1 植株的株高和单株穗数进行了考察,发现转绿型突变系及其相应的M4BC1 带叶色标记植株的农艺性状与相应的对照相仿。

【Abstract】 Several types of leaf color mutants were obtained by using 60 Co γ gamma rays treatment on dry seeds of maintainer long te fu B. In M 2 generation, the mutation frequencies of albino, yellowing and striping mutation lines at seedling stage were 0.347%, 0.041% and 0.031%, respectively. Investigations on their growth dynamics revealed that the average leaves and tillers of the whole yellowing and striping mutation lines were different from the original parent, but some mutants could recover to normal green at three leaf stage, and their leaves and tillers were similar to that of the control. In M 4BC 1 populations, there were two types of leaf phenotype segregation: normal green plants and the plants with leaf color marker, and their segregation were all about 1∶1. According to the characteristics of leaf color expression at seedling stage and later growth stage, these mutant lines in M 4 generation were classified into 6 types. Observations on the plant height, No. of panicles per plant, No. of total grains per panicle, seeds set per panicle and pollen fertility of M 4 mutant lines and plant height and No. of panicles of corresponding M 4BC 1 plants were conducted, the results showed that the agrinomical traits of those lines, which could recover to normal green, and corresponding M 4BC 1 plants with leaf color marker were similar to that of the corresponding control.

【基金】 国家科委项目;浙江省科委“九五”攻关资助
  • 【文献出处】 浙江大学学报(农业与生命科学版) ,JOURNAL OF ZHEJIANG AGRICULTURAL UNIVERSITY(AGRIC.& LIFE SCI.) , 编辑部邮箱 ,1999年06期
  • 【分类号】S124;S511.03
  • 【被引频次】94
  • 【下载频次】164
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