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转CMV CP基因番茄植株的抗病表现

Protection of transgenic tomato against CMV infection

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【作者】 施曼玲; 薛宝娣;

【Author】 SHI Man ling,Xue Bao di (1 Hangzhou Teachers’ College,Hangzhou 310012,Zhejiang,China; (1 Hangzhou Teachers’ College,Hangzhou 310012,Zhejiang,China; 2 Nanjing Agricultural University,Nanjing 210095,Jiangsu,China)2 Nanjing Agricultural University,N

【机构】 杭州师范学院生物系!浙江杭州310012; 南京农业大学植保系!江苏南京210095;

【摘要】 转CMVCP (Cucum berMosaic CoatProtein) 基因的番茄植株对CMV 侵染表现显著的抗性, 温室中接种的植株无症率为: R1 代94% , R2 代951% , R3 代954% , R4 代95% ; 大田中自然发病的植株无症率为: R2 代656% , R3 代769% 。在转基因的番茄植株中, 外源基因GUS(βglucuronidase)、CP和NPT Ⅱ(neom ycin phosphotransferase) 基因共同表达,连锁遗传,在后代按3∶1 或1∶1 比例进行分离。

【Abstract】 Transgenic tomato plants expressing the cucumber mosaic virus coat protein gene protected against CMV infection effectively.In greenhouse,protection effects of R 1,R 2,R 3 and R 4 progeny of self fertilized transgenic tomato against CMV infection were 94%,95.1%,95.4% and 95%,respectively.In the field,protection effects of R 2 and R 3 progeny were 65 5% and 76 9%.GUS,CP and NPT Ⅱ gene expressed together in transgenic R 0 tomato and its progeny.Exogenic genes in some transgenic plants segregated 3∶1,while others segregated 1∶1.

  • 【文献出处】 浙江农业科学 ,JOURNAL OF AGRICULTURAL SCIENCE , 编辑部邮箱 ,1999年06期
  • 【分类号】S432.1
  • 【被引频次】5
  • 【下载频次】36
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