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Unna氏多色性美蓝染液用于兔脑尼氏体染色

Use of Unna’s Polychromatic Methylene Blue Staining Solution in the Staining of Nissl Bodies in the Brain of Rabbits

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【作者】 胡满张海旺刘晓丹

【Author】 Hu Man et al.Department of Animal Medicine,ZJKAC,075131

【机构】 张家口农业高等专科学校动医系张家口农业高等专科学校动医系 075131075131075131

【摘要】 将Unna氏多色性美蓝染液用于兔脑尼氏体染色,摸索出一套合适的兔脑石蜡切片制作及尼氏体染色程序。所染切片尼氏体清晰,核仁明显,胞体轮廓分明,背景色浅,切片经久不褪色。较传统尼氏体染色方法具有易着色、不会过染、价廉易得和操作简便等优点。

【Abstract】 By appling unna’s polychromatic methylene blue staining solution to the stainingof Nissl bodies in the Brain of rabbits, a set of practical procedure of making the paraffin sec-tion and staining the Nissl bodies of the brain of rabbits was worked out. In the sectionsstained by this method, there were clear Nissl body, distinct nucleoli, obvious cell bodyoutline and light background. Besides, the sections do not fade for a long time. The methodhas the advartages of staining easily, being not overstained, obtaining easily, being cheapand operating simply and conveniently contrasting with traditional method of staining.

【关键词】 美蓝家兔尼氏体染色
【Key words】 Methylene blueRabbitNissl bodyStaining
【基金】 河北省教委科研资助
  • 【文献出处】 张家口农专学报 ,Journal of Zhangjiakou Teachers College , 编辑部邮箱 ,1999年03期
  • 【分类号】Q95-3
  • 【被引频次】1
  • 【下载频次】139
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