【摘要】 目的探讨Ｐ５３基因突变与眼睑恶性肿瘤发生的关系。方法采用链霉卵白素生物素（ｌａｂｅｌｅｄｓｔｒｅｐｔｒａｖｉｄｉｎｂｉｏｔｉｎ，ＬＳＡＢ）免疫组化方法检测１０２例眼睑恶性肿瘤组织Ｐ５３蛋白及增殖细胞核抗原（ｐｒｏｌｉｆｅｒａｔｉｎｇｃｅｌｎｕｃｌｅａｒａｎｔｉｇｅｎ，ＰＣＮＡ）表达情况。结果在正常眼睑皮肤、睑板腺及轻度不典型增生眼睑上皮中，未见Ｐ５３蛋白表达，而中、重度不典型增生，原位癌，浸润癌中，Ｐ５３蛋白阳性表达率逐渐增高，其ＰＣＮＡ指数也有增加趋势。３０／５３例基底细胞癌（ｂａｓａｌｃｅｌｃａｒｃｉｎｏｍａ，ＢＣＣ）、１６／３２例鳞状细胞癌（ｓｑｕａｍｏｕｓｃｅｌｃａｒｃｉｎｏｍａ，ＳＣＣ）、１０／１７例睑板腺癌中，Ｐ５３蛋白阳性表达；同时，随着肿瘤组织分化程度的降低，Ｐ５３蛋白阳性表达程度有逐渐增高的趋势。ＰＣＮＡ平均指数（ＰＩ）值在基底细胞癌、鳞状细胞癌，睑板腺癌中分别为３８．１±３１．１、３８．３±３０．１、４２．６±３２．０。在肿瘤的不同组织学分型上，ＰＣＮＡ平均指数随分化程度的降低有逐渐增高趋势，其差异有显著性（均Ｐ＜０．０５）。Ｐ５３蛋白阳性表达的肿瘤组织，其ＰＩ值均高于Ｐ５３蛋白阴性表?更多还原

【Abstract】 Objective To observe the relationship between P53 gene mutation and malignant eyelid tumor. Methods The expressions of P53 protein and proliferating cell nuclear antigen (PCNA) in 102 cases with malignant eyelid tumors were examined by labelled streptravidin biotin (LSAB) immunohistochemical technique. Results Although overexpression of P53 protein was not detected in normal eyelid cutaneous epithelium, meibomian gland and mild epithelial dysplasia, the positive rate of P53 increased in degree in moderate and severe dysplasia, carcinoma in situ and invasive tumors, and PCNA labeling index (PI) progressively increased too. Overexpression of P53 protein was detected in 30/53 cases of basal cell carcinoma (BCC), 16/32 cases of squamous cell carcinoma (SCC), 10/17 cases of meibomian gland carcinoma, and the intensity of P53 protein immunostaining increased with the lowering in the degree of histological differentiation. The mean PCNA labeling index was 38.1±31.1, 38.3±30.1, 42.6± 32.0 respectively in BCC, SCC and meibomian gland carcinoma. The PI value in tumor tissue with positive P53 protein expression was higher than that in tissues with its negative expression ( P <0.05). Conclusions The gene mutation of P53 protein might play an important role in the pathogenesis of malignant eyelid tumors, it may be one of the factors that can lead to abnormal hyperactivity of cell proliferation and it possibly can be a malignant sign in the progression of a tumor.更多还原