【摘要】 目的　了解超广谱β内酰胺酶（ Ｅ Ｓ Ｂ Ｌｓ） 细菌在医院分离及分布情况，以利于对 Ｅ Ｓ Ｂ Ｌｓ 细菌的监控和治疗。方法　用 Ｖ Ｉ Ｔ Ｅ Ｋ３２ 型全自动细菌分析系统药敏检测卡 Ｇ Ｎ Ｓ Ｎ Ｔ 进行细菌 Ｅ Ｓ Ｂ Ｌｓ 的测定。结果　在１３２ 株大肠埃希菌、１１９ 株肺炎克雷伯菌、６０ 株阴沟肠杆菌中 Ｅ Ｓ Ｂ Ｌｓ 的检出分别为２８８ ％ 、１９３ ％ 、５０ ％ ，而７３ 株绿脓假单胞菌未检到 Ｅ Ｓ Ｂ Ｌｓ 菌；各病区产 Ｅ Ｓ Ｂ Ｌｓ 菌的分离率以重症监护（ Ｉ Ｃ Ｕ） 病房最高（３３６ ％ ） ，其次为慢性病病房和干部病房（ 各为２００ ％ ） ，介入科病房（１９６ ％ ） ，呼吸科病房（１２９ ％ ） ，其他病房（３０ ％ ） ；产 Ｅ Ｓ Ｂ Ｌｓ 菌对亚胺培南全部敏感，对头孢哌酮／ 舒巴坦未发现有高耐株，中介占２３４ ％ （１５／６４） 。结论　 Ｖ Ｉ Ｔ Ｅ Ｋ３２ 型全自动细菌分析系统的 Ｇ Ｎ Ｓ Ｎ Ｔ 卡可正确检测产 Ｅ Ｓ Ｂ Ｌｓ 菌， Ｉ Ｃ Ｕ 病房是产 Ｅ Ｓ Ｂ Ｌｓ 菌的主要来源，对产 Ｅ Ｓ Ｂ Ｌｓ 菌的治疗以亚胺培南最佳，其次为高浓度的头孢哌酮／ 舒巴坦。更多还原

【Abstract】 Objective To investigate the distribution of producing extended spectrum β lactamase (ESBLs) bacteria in hospital for better control and treatment Methods Producing ESBLs bacteria were detected using GNS NT susceptibility card of Vitek 32 system Results Among 132 strains of Escherichia coli, 119 strains of Klebsiella pneumoniase and 60 strains of Enterobacter cloacae, producing ESBLs bacteria accounted for 28 8%, 19 3%, 5 0% No producing ESBLs strains were found in 73 strains of Pseudomenas aeruginosa The wards isolated rate of ESBLs, in the following order: 33 6% in ICU wards, 20 0% chronic diseases and veterans wards, 19 6% intervention wards was 12 9% respiratory system wards, and 3 0% others All producing ESBLs bacteria were susceptible to imipenem No strain was highly resistant to cefoperazone/sulbactam but 23 4% was intermediately resistant Conclusion Producing ESBLs bacteria could be detected accurately with GNS NT susceptibility card of Vitek 32 system The main source of producing ESBLs bacteria was ICU To kill these bacteria, imipenem can be used first,then cefoperazone/sulbactain更多还原