【摘要】 目的　研究Dow n 综合征动物模型16 三体和正常同窝鼠支配胃的神经发育。方法　采用16三体鼠培育,同窝鼠胚胎龄(em bryonic days, ED)13～18 天细胞遗传学分析,蛋白基因产物9.5(proteingene product 9.5, PGP9.5)免疫组化等方法对16 三体小鼠胃的神经发育进行了研究。结果　正常同窝鼠,胎龄13 天(ED13)来源于外胚层神经嵴的神经母细胞迁移并进驻胃壁;ED14 神经元发出突起,形成原始神经网络;ED15 形成简单排列的肌间神经丛,开始出现早期的神经节;ED16 有分布规则的肌间神经丛;ED17 神经母细胞进驻粘膜下层,形成粘膜下神经丛;ED18 完整的胃神经丛形成,即粘膜下浅、深神经丛和肌间神经丛。与正常同窝鼠比较,16 三体鼠胃神经系发育迟缓,ED14 胃壁始有散在分布神经元。此后,胃神经系的发育与分化均较正常延迟,至ED18 仅有肌间神经丛。根据胃神经系的发育程度和PGP9.5 免疫反应强度作半定量分析及秩和检验,16 三体鼠胃神经的发育明显落后于它们正常的同窝鼠,两者比较有显著性差异(P< 0.05)。结论　16 三体小鼠是公认的Dow n 综合征动物模型,它除了有多系统和多器官的畸型外,还发现有小鼠胃神经丛发育迟缓,粘膜下神经丛缺失。更多还原

【Abstract】 Objective To study the nervous development in the stomach of trisomy 16 mouse embryos and their normal littermates from embryonic days 13 18(ED 13 18) by using primary antibody against protein gene product (PGP) 9.5.Methods (A) trisomy 16 mouse breeding; (B) trisomy 16 mouse embryos were confirmed by examining their chromosomes;(C) PGP 9.5 immunohistochemical method.Results In normal littermates, the precursor cells from the neural crest migrated into stomach at ED13.Numerous nervous processes connected each other and formed early nervous networks at ED14. Original ganglia appeared in the muscular plexus with very simple arrangment at ED15.The developed myenteric and submucosal plexuses were found at ED16 and ED17 respectively.The myenteric plexus and the internal and external submucosal plexuses were differentiated at ED18.In comparison with the normal littermates the trisomy 16 mice’s stomach nervous development was much slower. Their immature neurons did not appear in the stomach until ED14; the development and differentiation of stomach nervous plexuses were delayed. A clear link was noted between the development of stomach plexuses and the density of PGP 9.5 immunostaining. A semiquantitative analysis and rank sum test of the data showed that the trisomy 16 mouse embryos were markedly retarded in nervous development of stomach, compared with their normal littermates ( P<0.05) . Conclusion Trisomy 16 mouse, as an animal model for Down syndrome,has abnormality not only in several systems and organs but also in stomach innervation. This is the first report on delayed nervous development in the stomach of trisomy 16 mice.更多还原