【摘要】 目的　观察再生障碍性贫血（再障） 患者骨髓造血干／祖细胞ｃｋｉｔ 受体（ＣＤ１１７） 的表达，以了解再障造血功能衰竭与ｃｋｉｔ 受体水平间的关系。方法　采用免疫荧光法及流式细胞技术检测２０例再障患者骨髓单个核细胞（ＭＮＣ） 和ＣＤ３４＋ 细胞ＣＤ１１７ 的表达水平。结果　①ＭＮＣＣＤ１１７ 表达率慢性再障（ＣＡＡ） 组为（２ ．０８ ±１．９６） ％ 、重型再障（ＳＡＡ）组为（０．８９ ±０．３２） ％ 、正常对照组和增生性贫血组分别为（０ ．４５ ±０．１６） ％ 和（０．６７±０ ．３９） ％ ，ＣＡＡ 组和ＳＡＡ 组明显高于正常对照组和增生性贫血组（ Ｐ＜０－０５） ；②ＣＤ３４ ＣＤ１１７双阳性细胞百分率，ＣＡＡ 组、ＳＡＡ 组、正常对照组和增生性贫血组分别为（０ ．８７ ±０－５７） ％ 、（０．６５±０．４１）％ 、（０．４１±０ ．１５）％ 和（０．４６±０．２６）％ ，ＣＡＡ 组和ＳＡＡ 组明显高于正常对照组和增生性贫血组（ Ｐ＜ ０．０５）；③ＣＤ３４＋ 细胞群ＣＤ１１７ 的表达，ＣＡＡ 组、ＳＡＡ 组、正常对照组和增生性贫血组分别为（７１ ．６±１２．４）％ 、（８８．９ ±２３．５） ％ 、（４６ ．３±９ ．７） ％ 和（４１．８ ±?更多还原

【Abstract】 Objective To explore the expression of c kit receptor(CD 117 ) on the hemopoietic stem/precursor cells of aplastic anemia patients and its role in the genesis of aplastic hematopoiesis failure.Methods c kit expression on the bone marrow mononuclear cells(MNC) and CD 34 positive cells from 11 chronic aplastic anemia(CAA),9 severe aplastic anemia(SAA),10 non aplastic anemia(NAA) patients (5 iron deficiency anemia and 5 autoimmune hemolytic anemia) and 5 healthy controls was measured with flow cytometric immunofluorescence assay and anti CD 117 monoclonal antibody.Results The CD 117 positive rates of MNC in CAA,SAA,NAA and control groups were (2.08±1.96)%, (0.89±0.32)%, (0.67±0.39)% and (0.45±0.16)%,respectively,and the rates were significantly higher in CAA and SAA groups than in NAA and control groups(P<0.05).The CD 117 and CD 34 double positive cells of MNC in CAA,SAA,NAA and control groups were (0.87±0.57)%,(0.65±0 41)%,(0.46±0.26)% and (0.41±0.15)%,respectively,and were significantly higher in CAA and SAA groups than in NAA and control groups (P<0.05).The CD 117 positive cells of CD 34 positive cells were also higher in CAA[(71.6±12.4)%] and SAA[(88.9±23.5)%] groups than in NAA[(41.8±10.3)%] and control[(46 3±9.7)%] groups (P<0.01).Conclusion Hemopoietic stem/precursor cells of aplastic anemia patients expressed more c kit receptors than those of NAA patients and healthy controls.The hematopoiesis failure occurred in aplastic anemia is not attributed to low expression of c kit receptor on the hemopoietic stem/precursor cells.更多还原