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健康成人血小板膜糖蛋白的表达特征
Expression patterns of platelet membrane glycoprotein in healthy adults
【摘要】 目的 观察健康成人血小板6 种膜糖蛋白( G P) 在不同性别、不同年龄,静息状态与活化状态时的表达特征。方法 用流式细胞仪测定静息状态下及用凝血酶受体活化肽( T R A P) 激活的血小板所结合单抗的分子数。结果 男性静息血小板和活化血小板中 G P Ⅱb/ Ⅲa 和 G P Ⅲa 显示随年龄减少现象,在静息血小板中 G P Ⅱb/ Ⅲa 和 G PⅢa 值与年龄呈负相关,r 值分别为 - 0 .409 和- 0 .534 ,其 P 值均< 0 .05 ;而女性中未见到类似结果。 T R A P 激活后 G P Ib 在血小板表面表达下降,而 G P Ⅱb/Ⅲa 、Ⅲa 、 P选择素均增高,其中 P选择素在激活后的升高值与激活前的基础值呈负相关。静息血小板的 G PⅠb 分子数约为30 000 ,它与 G P Ⅴ的比值约3∶1 。结论 健康人血小板 G P表达存在性别和年龄差异。
【Abstract】 Objective To study the expression patterns of 6 kinds of platelet membrane glycoprotein(GP) in resting and activated platelets of healthy adults. Methods The molecule numbers of platelet GP Ⅲa,Ⅱb/Ⅲa,Ⅰb,Ⅸ,Ⅴ and P selectin on resting platelets or platelets activated by thrombin receptor activating peptide (TRAP) in 60 healthy adults (male/female=1/1) were detected by flow cytometry. Results The expression of GPⅡb/Ⅲa and GPⅢa seemed to decrease with the increase of age in the resting and activated platelets of males. The coefficients of correlation(r) in resting platelets were -0.409 and -0.534 (P<0.05), respectively;whereas this phenomenon were not found in females. The expression of GPⅠb was reduced while the expressions of GPⅡb/Ⅲa, Ⅲa , P selectin increased after the platelets were activated by TRAP. There was a negative relationship between resting and activated platelets in the expression of P selectin. The molecule number of GPⅠb on the surface of resting platelet was about 30?000, the ratio of GPⅠb to GPⅤ was 3∶1. Conclusion It seems that there exists some difference with age and sex in the expression of platelet membrane glycoproteins in healthy persons.
- 【文献出处】 中华血液学杂志 ,CHINESE JOURNAL OF HEMATOLOGY , 编辑部邮箱 ,1999年09期
- 【分类号】R329326
- 【被引频次】17
- 【下载频次】61