【摘要】 目的探讨WAF1表达水平对神经母细胞瘤（NB）发生发展的影响。方法应用免疫组化技术对20例NB标本进行检测。结果18例可见WAF1表达阳性细胞，表达指数0～45.6％。神经节神经母细胞瘤表达指数显著高于NB（P＜0．05），预后好型表达指数显著高于预后差型（P＜0．01）。有丝分裂核碎裂指数（MKI）、瘤体直径与之呈负相关，术后存活时间与之呈正相关（r分别为-0．89、－0．49和0．47）。表达指数＞20％者MKI、瘤体直径均显著低于表达指数＜20％者（P均＜0.01），术后存活时间则明显长于后者（P＜0．05）。结论WAF1表达水平与NB发生发展关系密切。WAF1表达水平高时，能抑制NB细胞增殖，预后好；反之，肿瘤生长快，预后不良。更多还原

【Abstract】 Objective To evaluate the relationship between WAF1 gene product (p21 proein) and neuroblastoma (NB). Methods Twenty peicimens of NB were immunostained for WAF1 gene product.Results OUt of 20 specimens,18 were positive for WAF1 gene product with expression index of 0 -45. 6%. The expression in ganglioneuroblastoma was stronger than NB (P<0.05). The expression in NB specimens with good prognostic index was stronger than those with poor prognostic index (P <0.01).Negative correlation was found between mitotic karyorrhexis index (MKI) and tumor diameters (r =- 0. 89, r = 0. 49 respectively). There was positive correlation between survival time and the expres-sion index (r =0. 47). The MKI and the tumor diameter were significantly lower in specimens with ex-pression index >20% than the specimens with expression index < 20% (P<0.01). The post-operative survival time was significantly longer in the former group than the latter (P< 0.05). Conclusions The ex-prsion of WAF1 gene is implicated in the development of NB; higher expression is associated with better prognosis and vice versa.[更多还原