【摘要】 目的　研制识别癌胚抗原的基因工程单链抗体，使其能够应用于肿瘤的免疫诊断和治疗。方法　利用ＲＴＰＣＲ 方法扩增抗体可变区基因并表达于噬菌体表面。通过免疫筛选获得癌胚抗原特异单链抗体ＣＬ３ｓｃＦｖ 。结果　ＥＬＩＳＡ 和免疫组化均显示抗体ＣＬ３ｓｃＦｖ 能够结合ＣＥＡ，而不与正常胃肠道组织起反应。结论　 该抗体将成为胃肠道恶性肿瘤辅助诊断和治疗的有效试剂。更多还原

【Abstract】 Objective To produce a bio engineered singlechain antibody to carcinoembryonic antigen that can be used in tumor immunodiagnosis and therapy. Methods The genes encoding antibody variable regions were cloned by RT PCR from hybridoma cells and expressed on bacterial phage surface. By three panning rounds, we have obtained a single chain antibody that was specific for carcinoembryonic antigen, referred as CL 3 scFv. Results ELISA and immunohistochemistry showed that the antibody could be bound to carcinoembryonic antigen as well as to tumor cells in colon and gastric carcinomas but not normal cells. Conclusion The antibody CL 3 scFv will be a reagent for the diagnosis and therapy of gastrointestinal cancer.更多还原