【摘要】 目的　 寻求Ｏ１３９ 型霍乱弧菌菌毛提取纯化的最适方法和其疫苗研制。方法　ＳＤＳＰＡＧＥ，斑点酶免疫（ＤＢＩ） 检测和ＢＡＥＬＩＳＡ 法。采用ＰＥＬＡ 材料包封菌毛，成为缓释微球疫苗。结果电泳均存在相对分子质量为２０ ．５ ×１０３ 的条带；ＤＢＩ结果为阳性。小鼠血清ＩｇＧ 以皮下ＭＳＴＣＰ 组最高（１∶７ ６８０） ，唾液中的ｓＩｇＡ 以口服ＭＳＴＣＰ 组最高（１∶６０） 。保护力试验中口服组的保护力约为３０ ％ ～４０ ％ ，而皮下组为７０ ％ ～９０ ％ 。结论　 口服组主要诱导粘膜免疫，皮下组主要诱导全身性免疫。ＴＣＰ 是霍乱弧菌的共同抗原之一，可作为霍乱疫苗的候选抗原。更多还原

【Abstract】 Objective To study the purification of V. cholera O139 pili and vaccine research. Methods The toxin coregulated pili (TCP) purified was measured with sodium dodecyl sulfatepoly acrylamide gel electrophoresis (SDS PAGE) and its molecular weight was about 20.5 kD; TCP was identified with dot blot immunoassay. Sera IgG and salivary sIgA after immunization were assayed with BA ELISA. Results (1)IgG titer of subcutaneous MS TCP injection group was the highest among the test groups.(2) sIgA titer of oral MS TCP group was the highest among the test groups. These results suggested that mucosal immune responses were mainly induced in oral immunizaton groups, systemic immune responses were chiefly induced in subcutaneous injection groups. Animal protection test indicated that immunized mice had acquired resistance to challenging of V.cholerae. Protective ratio of oral TCP groups was about 30%～40%,70%～90% in subcutaneous TCP group when challenged by O139 biotype M045 strain. Conclusion According to our experimental results, TCP is one of the common antigens of Vibrio cholerae, and promises to be a candidate antigen of V.cholerae vaccine.更多还原