【摘要】 目的　通过研究骨肉瘤中p16及其相关因子表达情况 ,了解其在骨肉瘤中的异常程度及其功能缺失的实质。　方法　用Western方法检测骨肉瘤组织和不同周期骨肉瘤细胞p16蛋白及其相关因子的定量表达 ,用免疫荧光方法检测不同周期骨肉瘤细胞p16的定位表达情况。　结果　12例骨肉瘤组织中 10例有p16蛋白表达 ,所有标本均有pRb表达 ,但其中 8例以高度磷酸化为主 ;通过骨肉瘤细胞系证明p16在G1期表达最低 ,随周期进程而增加 ,p16蛋白在G1期位于胞质 ,在G2期入核。　结论　骨肉瘤组织中p16蛋白表达率为 83 3 % ,大部分骨肉瘤虽然有p16蛋白的表达 ,但其不足以抑制CyclinD1/CDK4的活性和pRb的磷酸化 ,细胞恶性度得不到遏制 ;p16功能不足可能与其G1期表达低有关。更多还原

【Abstract】 Objective To study the expression and regulation of p16 and related factors in osteosarcoma. Methods The levels of p16 protein and related factors were explored by Western blot analysis in 12 cases of osteosarcoma tissues and in cells of different phases of osteosarcoma cell line OS732. The status of p16 protein in osteosarcoma cells was revealed by immunofluorescence analysis. Results p16 protein was detected in 10 of 12 osteosarcoma tissues. pRb was found in all of the specimens, but 8 of them were hyperphosphorylated. p16 protein was the least in G1 phase. Localized in cytoplasm during G1 phase, p16 protein gathered around nuclear envolope in S phase and entered the nucleus in G2 phase. Conclusions The expression rate of p16 protein was 83 3% in osteosarcoma tissues. The expression of p16 protein was unable to inhibit the phosphorylation of pRb in most osteosarcomas; p16 protein was extremely low in G1 phase, presumably it was sufficient to inhibit the phosphorylation of pRb, causing abnormal proliferation of the cells.更多还原