【摘要】 目的　为了减少肝细胞在微囊化过程中的缺氧损伤， 研究在缺氧状态下果糖对肝细胞的保护作用。方法　将ＳＤ大鼠分为三组：Ａ 组， 细胞分离的灌流液和消化液中均加果糖２０ ｍ ｍｏｌ／Ｌ，Ｂ、Ｃ两组不加果糖； 细胞分离及微囊化后， 检测细胞的活率； 微囊化肝细胞行体外培养，Ｃ组培养液中加果糖１０ ｍ ｍｏｌ／Ｌ，Ａ、Ｂ两组不加。测定三组肝细胞的乳酸脱氢酶（ＬＤＨ） 漏出率（ ％ ） 及培养上清液中的总蛋白质。结果　Ａ组的细胞活率显著高于Ｂ、Ｃ 两组（ Ｐ＜ ０ ．０１） ， 而Ａ 组的ＬＤＨ 漏出率显著低于Ｂ、Ｃ组（ Ｐ＜ ０ ．０１） ，Ａ 组蛋白质合成功能的恢复显著优于Ｂ、Ｃ 组（ Ｐ＜ ０ ．０１） 。结论　果糖在缺氧状态下能有效延缓肝细胞的缺氧损伤， 但应在缺氧状态发生之前给予才能发挥作用。更多还原

【Abstract】 Objective To study the protective effect of fructose with regard to hypoxia induced hepatocytes injury during the long microencapsulation periods. Methods Eighteen SD rats were divided into three groups, 6 rats each. In group A, fructose was added into the perfusion solutions (20?mmol/L). There was no fructose in group B and C. After isolation and microencapsulation, the viability was tested. In vitro culture, fructose (10?mmol/L) was added in culture medium in group C, but not in groups A and B. LDH leakage and TP of culture were examined.Results The survival of hepatocytes in group A was obviously higher than in groups B and C ( P < 0.01 ). The LDH leakage in group A was significantly lower than in groups B and C ( P < 0.01 ).Conclusion Fructose could protect hepatocytes against hypoxia induced cell damage during microencapsulation, but cells should be pre treated with fructose before hypoxia.更多还原