【摘要】 目的探讨银屑病患者白介素8(IL 8)、人类白细胞抗原DR(HLA DR)、磷脂酶A2(PLA2)、酸性α 乙酸萘酯酶(ANAE)、表皮生长因子受体基因(EGFRmRNA)、c fos基因(c fosmRNA)在皮损的表达定位。方法对20例银屑病患者皮损和10例正常人的皮肤标本进行了免疫组化、组化和原位杂交技术检测。结果IL 8免疫反应主要定位于表皮的基层、棘细胞层和颗粒层、血管内皮细胞及间质细胞 ;人类白细胞抗原DR免疫反应及ANAE分型染色主要定位于浸润细胞[包括单核巨噬细胞及T辅助淋巴细胞(TH细胞)]和血管内皮细胞 ;PLA2 活性主要定位于表皮细胞的胞膜及角层和间质细胞 ;以上观察指标在皮损区反应均较强或反应细胞较多 ;c fosmRNA的杂交信号皮损区弱于对照区 ;EGFRmRNA在皮损区杂交信号虽较强 ,但与对照组相比差异不显著。结论IL 8及其介导的免疫炎症反应在银屑病发病中起着十分重要的作用 ,并使PLA2活性增强 ,淋巴细胞激活。c fos原癌基因在增殖的角质形成细胞中呈弱表达 ,提示其终末分化较差。而EGFRmRNA的表达强度则与银屑病无显著相关关系。而IL 8与HLA DR和TH细胞之间呈明显正相关(P<0.01)。更多还原

【Abstract】 Objective To study the localization of interleukin 8 (IL 8), HLA DR, phospholipase A2 (PLA2), acid α naphthyl acetate esterase (ANAE), epidermal growth factor receptor mRNA (EGFR mRNA), c fos mRNA in psoriatic skin. Methods Skin lesions of 20 psoriatic cases who received no treatment in previous three months and skin samples of 10 controls were detected by histochemistry, immunohistochemistry and in situ hybridization technique. Results IL 8 was mainly localized at the basal cell layer, spinous cell layer and granular cell layer of the epidermis, the endothelium of the blood vessels and interstitial cells; HLA DR and ANAE were mainly localized at the infiltrating immune cells in dermis (including TH cells and mononuclear phagocytes) and the endothelial cells of the blood vessels; PLA2 reaction was mainly seen at the cytomembrane of keratinocytes in deeper epidermal layers, cornified layer and dermal interstitial cells. All above mentioned positive reactions were more intense, or there were more positive cells in psoriatic lesions than those in controls. c fos mRNA signal were weaker in lesions than that in controls. EGFR mRNA signal was more intense in lesions, but there was no significant difference between those in the lesions and in controls. Conclusion The results suggest that IL 8 and its mediated inflammatory reactions might play an important role in the development of psoriasis, and it also enhances PLA2 activity and activates lymphocytes. c fos protooncogene is weakly expressed in proliferative keratinocytes. No distinctive correlation between EGFR mRNA and the development of psoriasis is found.更多还原