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酪蛋白磷肽的制备
PREPARATION OF CASEIN PHOSPHOPEPTIDES
【摘要】 报道枯草杆菌蛋白酶水解酪蛋白的最适酶反应系统。当以CaCl2-乙醇沉淀法沉淀磷肽时,比较了不同pH条件对磷肽产品得率、纯度、N/P比及含磷量的影响。
【Abstract】 The optimal enzymatic reaction system for the hydrolysis of casein with Alcalase was studied.When the product containing phosphopeptides was precipitated by the CaCl 2/ethanol process,the effects of pH on yield,purity,N/P ratio and P content were compared separatively.
【关键词】 磷肽;
酪蛋白;
枯草杆菌蛋白酶;
金属螯合亲和柱色谱;
【Key words】 casein; phosphopeptide; Alcalase; metal chelate affinity chromatography;
【Key words】 casein; phosphopeptide; Alcalase; metal chelate affinity chromatography;
- 【文献出处】 中国医药工业杂志 ,CHINESE JOURNAL OF PHARMACEUTICALS , 编辑部邮箱 ,1999年03期
- 【分类号】TQ464.7
- 【下载频次】62