【摘要】 目的研究血管紧张素转换酶抑制剂（ＡＣＥＩ）对糖尿病大鼠肾皮质细胞膜胰岛素受体表达的调节。方法实验大鼠随机分三组：单侧肾切除组（Ｃ组，６例）；糖尿病组（Ｄ组，７例）及糖尿病苯那普利治疗组（ＤＢ组，７例）。观察四周后各组血糖、血胰岛素及血肌酐水平和体重、肾重及肾重／体重之比的变化，测定血浆、肾皮质及髓质血管紧张素转换酶（ＡＣＥ）活性，采用Ｗｅｓｔｅｒｎ杂交分析肾皮质细胞膜胰岛素受体蛋白表达。结果苯那普利治疗四周后能够改善糖尿病大鼠血糖、血胰岛素及血肌酐水平的异常，对糖尿病肾脏肥大有显著抑制作用，对血浆、肾皮质及髓质ＡＣＥ活性抑制分别达９２．００％，８８．７７％与７３．４０％。肾皮质细胞膜胰岛素受体蛋白表达比对照组增加约２．１０倍，苯那普利治疗四周后对此有明显的下调作用。结论苯那普利可下调糖尿病状态下肾皮质细胞膜胰岛素受体蛋白高表达，这可能是其对糖尿病肾脏保护作用的重要机制之一。更多还原

【Abstract】 Objective To investigate into the regulation of renal cortex cell membranes insulin receptor indiabeticratsbyangiotensinconvertingenzymeinhibitor(ACEI). Methods The rats were randomly divided into following groups: uninephrectomized rats (group C, n=6), streptozotocin induced diabetic rats (group D, n=7) and diabetic rats treated with benazepril (an ACEI), (group DB, n=7). Blood glucose, serum insulin, serum creatinine level as well as body weight, kidney weight and kidney weight/body weight were observed after 4 weeks of study. ACE activities in plasma, renal cortex and medulla were measured by fluorimetric assay. The expression of renal cortex cell membranes insulin receptor were determined by Western blot analysis. Results After 4 weeks of treatment, benazepril could ameliorate hyperglycemia, decrease serum insulin and increase serum creatinine level. It could also significantly supress kidney hypertrophy in diabetic state. ACE activities in plasma, renalcortexandmedulla were reduced by approximately 92.00%, 88.77% and 73.40%, respectively. Western blot analysis showed that the expression of renal cortex cell membranes insulin receptor was increased by about 2.10 folds in diabetic rats, however, benazepril might significantly down regulate its expression. Conclusion Benazepril may down regulate the increased expression of renal cortex cell membranes insulin receptor protein in diabetic rats, which may be one of the important mechanism of its nephroprotection.更多还原