【摘要】 目的　研究Ｂａｘ 蛋白和热休克蛋白（ＨＳＰ）７０ 在完全弗氏佐剂（ＣＦＡ）预防ＮＯＤ 鼠胰岛炎和糖尿病中作用及意义。方法　给４ 周龄雌性ＮＯＤ 鼠后脚板注射５０ μｌ ＣＦＡ 和生理盐水（ＮＳ组） ，于３０ 周龄或糖尿病发生时处死，胰腺组织置１０ ％ 中性福尔马林固定后常规制成石蜡切片，ＨＥ染色后行光镜下胰岛炎评分，ＡＢＣ免疫组化染色Ｂａｘ 蛋白和ＨＳＰ７０ 并光镜下计数。结果　ＣＦＡ 组鼠胰岛内炎计数和胰岛炎评分均数均明显低于ＮＳ组鼠（Ｐ＜ ０．０１）；ＣＦＡ 组鼠无１ 只发生糖尿病，ＮＳ组鼠中有３ 只（６０％ ）发生糖尿病；ＣＦＡ 组鼠胰岛细胞Ｂａｘ 蛋白阳性率明显低于ＮＳ组鼠，而ＨＳＰ７０ 阳性率明显高于ＮＳ组鼠，均有极显著性差异（Ｐ＜ ０．０１） ； 胰岛炎分值与Ｂａｘ 蛋白阳性率呈正相关（ｒ＝０．６１３） ，与ＨＳＰ７０ 阳性率呈负相关（ｒ＝ － ０．９１３）。结论　ＣＦＡ 能减轻ＮＯＤ 鼠胰岛炎发生程度和预防糖尿病发生，ＣＦＡ能抑制胰岛细胞Ｂａｘ 蛋白表达和诱导胰岛细胞ＨＳＰ７０ 表达。更多还原

【Abstract】 Objective To study the effects and significance of Bax protein and heat shock protein 70 (HSP70) in prevention of insulitis and diabetes by complete Freund’s adjuvant (CFA) in female NOD mice. Methods 5 female NOD mice of CFA treated group were injected into the pad of hind foot with 50μl CFA and 5 female NOD mice of control group with 50μl normal saline (NS) at 4 weeks of age. They were sacrificed at 30th week of age or when diabetes occurred. The pancreatic tissues were routinely processed and HE stained and scoring of insulitis was done under optical microscopy. The Bax oncoprotein and HSP70 were stained with ABC immunohistochemical method and the counts of positive cells in islets were finished under high power field. Results The incidence of in insulitis and the average score of insulitis were significantly lower in CFA treated mice than that in NS treated mice (P<0.01). None of CFA treated mice and 3 out of 5 NS treated mice developed diabetes. The positive rate of Bax oncoprotein in islet cells was significantly lower in CFA treated mice than that in NS treated mice, the positive rate of HSP70 in islet cells was significantly higher in CFA treated mice than that in NS treated mice. Positive correlation was found between the positive rate of Bax oncoprotein in islet cells and the score of insulitis (r=0.613), and negative correlation between positive rate of HSP70 in islet cells and the score of insulitis (r=-0.913) was also revealed. Conclusion CFA can reduce insulitis severity and decrease incidence of diabetes in NOD female mice. CFA is able to inhibit the expression of Bax oncoprotein and induce the expression of HSP70 in islet cells.更多还原