【摘要】 目的　探讨９ 日龄雄激素致不孕大鼠（９ｄＡＳＲ）肥胖无排卵机制及滋肾阴药的作用。方法　给出生９ 日龄ＳＤ雌性大鼠一次性皮下注射丙酸睾丸酮，建立９ｄＡＳＲ模型。检测雌激素受体（ＥＲ） 和神经肽Ｙ（ＮＰＹ） 神经元共存情况；检测９ｄＡＳＲ 下丘脑ＥＲ、ＮＰＹ 及促性腺激素释放激素（ＧｎＲＨ） 含量变化；测定血雌二醇（Ｅ２）、睾酮（Ｔ） 、瘦素、促卵泡激素（ＦＳＨ）、促黄体激素（ＬＨ） 的变化。结果　ＡＳＲ呈能量失衡致肥胖状态，血Ｅ２ 、Ｔ、瘦素水平明显增加（ Ｐ＜０ ．０１），ＦＳＨ、ＬＨ 明显低下（Ｐ＜０．０１），下丘脑ＥＲ存在于ＮＰＹ 神经元上，其数目减少，ＮＰＹ 表达增强，ＧｎＲＨ 分泌下降。ＮＰＹ 与ＥＲ或ＧｎＲＨ 均呈负相关（ｒ＝ － ０ ．７１０４，Ｐ＜ ０ ．０１；ｒ＝ － ０ ．８８０２ ，Ｐ＜ ０．０１）。瘦素与体重呈正相关（ｒ＝０．８９７７ ，Ｐ＜０ ．０１），与ＦＳＨ、ＬＨ 呈负相关（ｒ＝ － ０ ．７５１７ ，Ｐ＜ ０ ．０１；ｒ＝ － ０ ．８４４４ ，Ｐ＜ ０．０１）。用滋肾阴药治疗后，各神经内分泌代谢指标均恢复正常。结论　９ｄＡＳＲ模型有神经内分泌代谢失调而致肥胖并引起低促性腺激素性性腺功能低下性无排卵，滋肾?更多还原

【Abstract】 Objective To explore the mechanism of obesity and chronic anovulation in androgen sterilized rat at age of 9 days (9d ASR) and the effect of herbs of tonifying Kidney yin. Methods 9d ASR model was established by injecting testosterone propionate subcutaneously to SD female rat at age of 9 days. Double immunofluorescent staining was carried out on sections through arcuate nucleus (ARC) to determine whether neuropeptide Y (NPY) containing neurons expressed estrogen receptors (ER). The immunoreactive amounts of ER, NPY neurons and gonadotropin releasing hormone (GnRH) fibers of hypothalamic ARC median eminence (ME) in 9d ASR were studied with immunohistochemistry. Serum estrogen (E 2), testosterone (T), leptin, follicular stimulating hormone (FSH) and luteinizing hormone (LH) were measured with radioimmunoassay. All parameters were reevaluated after administration of herbs to 9d ASR. Results Estrogen receptors were predominantly distributed in the cytoplasm of NPY containing neurons as well as in the nucleus and axons of NPY synthesizing neurons. Compared with normal group, ASRs were characterized by high metabolism rate, obesity, increase in hypothalamic NPY expression (P<0.01), decrease in numbers of ER and GnRH secretion (P<0.01). Levels of serum E 2, T and leptin were significantly higher and FSH and LH levels significantly lower in 9d ASRthaninnormalrats (P<0.01). Significantly negative correlation between NPY and ER or GnRH (r=0.7104, P<0.01; r=-0.8802, P<0.01, respectively) were observed, andsowerethecorrelationsbetweenleptinandFSHor LH (r=-0.7517, P<0.01; r=-0.8444, P<0.01, respectively). Serum leptin showed positive correlation with body weight in 9d ASR (r=0.8977, P<0.01). These dramaticneuroendocrine metabolicchanges becamenormal after feeding 9d ASR with herbs. Conclusions Obesity and chronic anovulation coexist in 9d ASR. Elevated E 2 levels may down regulate the hypothalamic ER oriented NPY neurons, followed by the release of NPY and suppression of GnRH. Effect of the herbs may be involved in reducing body weight and inducing ovulation.更多还原