【摘要】 目的　探讨卡托普利（ＣＡＰ）对家兔肾上腹主动脉阻断致急性肾缺血／再灌注损伤（ＡＲＩＲＩ）的保护效果。方法　于肾上阻断腹主动脉血流３０ｍｉｎ再灌注１８０ｍｉｎ的方法制成双侧ＡＲＩＲＩ动物模型，将２４只家兔随机等分为假手术组（Ａ组）、缺血再灌注组（Ｂ组）和ＣＡＰ治疗组（Ｃ组）。Ｃ组于阻断主动脉前 ５ｍｉｎ静注 ＣＡＰ２ｍｇ· ｋｇ^－１，继之持续输注 １５ｍｉｎ ＣＡＰ ０．５ｍｇ· ｋｇ^－１· ｈ^－１。 Ａ、Ｂ组以等容积生理盐水取代ＣＡＰ，Ａ组不阻断主动脉血流。动态检测血中ＢＵＮ、Ｃｒ、ＳＯＤ、ＭＤＡ、ＡＴ－Ⅱ，尿β_２－ＭＧ变化，以及肾皮质中ＳＯＤ、ＭＤＡ、ＡＴ－Ⅱ和肾组织形态学改变。结果　Ｃ组在再灌期血和肾皮质中ＭＤＡ、ＡＴ－Ⅱ浓度明显低于Ｂ组（Ｐ＜０．０１），ＳＯＤ活性显著高于Ｂ组（Ｐ＜０．０１），血ＢＵＮ、Ｃｒ及尿中β２－ＭＧ含量明显低于Ｂ组（Ｐ＜０．０５，＜０．０１）。Ｃ组光镜下肾小管损伤Ｐａｌｌｅｒ评分明显低于Ｂ组（２０．５０±７．５６ｖｓ ８２．５０±１６．６９，Ｐ＜０．０５），Ｂ组电镜见明显急性肾小管损伤及坏死，而Ｃ组肾小管损伤轻微。结论ＣＡＰ对家兔肾上腹主动脉阻断所致ＡＲＩＲＩ有良好的保护?更多还原

【Abstract】 Objective To evaluate the protective effect of captopril（CAP） on acute renal ischemia-reperfusion injury in rabbits. Methods Model of acute renal ischemia-reperfusion injury was induced with the right suprarenal abdominal aorta being occlused for 30min then unclamped for 180 min. 24 rabbits were randomly divided into false operating group （A, n = 8）, ischemia-reperfusion group （B, n = 8）, and CAP-group （C, n = 8）. In group C, CAP 2mg.kg^-1 was given intravenously 5min prior to clamping and infused at a rate of 0.5mg. kg（-1）. h（-1） for 15min. Other groups of rabbits received normal saline as controls,but the abdominal aorta was not occlused in group A. The parameters, including BUN, Cr, SOD, MDA, AT-Ⅱ and β₂-MG, were observed before, during ischemia and after reperfusion. The kidney specimens were examined using light and electron microscopy’ Results In group C renal cortex and serum MDA,AT-Ⅱ concentration after reperfusion were significantly decreased （P＜0.01）,SOD activity increased significantly （P<0.01） and serum BUN,Cr,urine β₂-MG levels decreased markedly compared with those in group B （P<0. 05 or P<0.01 ）’ The Paller’s score for acute renal tubular injury was evidently lower in group C than that of group B （25. 00±7. 56 vs 82. 50±16. 69,P<0.05）’ Under electron microscopy,renal pathology associated with acute tubular injury and necrosis were observed in group B, meanwhile, it was obviously alleviated in group C’ Conclusion Captopril can effectively protect rabbit kidneys against ischemia-reperfusion injury during suprarenal abdominal aortic crossclaming.更多还原