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尿沉渣微卫星检测在膀胱移行细胞癌诊断中的价值
Microsatellite analysis of urine in the detection of transitional cell carcinoma of bladder
【摘要】 目的 探讨微卫星不稳定性( MSI) 检测在膀胱移行细胞癌(TCC) 诊断中的价值。 方法 采用PCR 扩增、聚丙烯酰胺凝胶电泳、银染等技术。选用了20 种微卫星标记物对50 例TCC 进行分析( 每例均包括血、尿及肿瘤组织3 种标本) 。 结果 50 例尿标本中47 例发生MSI,阳性率为94 % ,每例患者尿中微卫星改变均与原发肿瘤标本一致。 结论 尿沉渣MSI检测技术是诊断膀胱肿瘤敏感有效的、无损伤的方法之一。
【Abstract】 Objective To investigate the role of MSI in urine in TCC of bladder. Methods MSI assay was carried out for blood,urine and tumor samples of 50 cases of TCC of bladder using PCR with 20 microsatellite loci. Results MSI was positive in urine samples of 47 of 50 patients (94%) and the genetic change in urine DNA was identical to that in the primary tumor in all. Conclusions Microsatellite analysis of urine sediment is a sensitive,effective and nontraumatic means for the early detection of bladder cancer.
- 【文献出处】 中华泌尿外科杂志 ,CHINESE JOURNAL OF UROLOGY , 编辑部邮箱 ,1999年10期
- 【分类号】R737.140.4
- 【被引频次】2
- 【下载频次】30