节点文献

组织工程学材料替代输尿管缺损的动物实验及临床应用

Histo engineering material for the replacement of ureter

  • 推荐 CAJ下载
  • PDF下载
  • 不支持迅雷等下载工具,请取消加速工具后下载。

【作者】 刘流梁德江申鹏飞胡杰满立波戴群生何峰黄广林王海

【Author】 LIU Liu ,LIANG Dejiang,SHEN Pengfei,et al.  Department of Urology,Beijing Ji Shui Tan Hospital,Beijing 100035

【机构】 北京积水潭医院泌尿外科湖南医科大学湘雅医院泌尿外科杰雅莱福生物技术公司技术部

【摘要】 目的 寻求理想的输尿管替代材料。 方法 将20 只兔双侧输尿管脱细胞处理后制成输尿管细胞外基质(ECM) ;另60 只兔分为3 组:输尿管ECM 移植组( 实验组) ,对照组1 及对照组2 。 结果 实验组术后组织学检查显示1 周时ECM 边缘部腔内面已见移行上皮细胞,管壁有肌细胞长入。6 周时取材ECM 区已修复近正常输尿管组织结构。12 、24 周时IVU 检查;输尿管显影良好,通畅。对照组1 术后7 ~10 天,移植的异体输尿管均坏死。对照组2 输尿管缺损区6 周时均被纤维包裹而明显增粗。在实验取得满意结果的基础上成功应用于临床。 结论 异体脱细胞输尿管细胞外基质为理想的输尿管替代材料。

【Abstract】 Objective To experiment the possibility of allodecellular extracellular matrix for the replacement of ureter. Methods Extracellular matrix was obtained from the ureters of 20 rabbits by decellularization process.60 experimental rabbits were used and a 4cm segment of left ureter was resected in each.In the experimental group of 20 rabbits,the resected ureter was replaced by the allo ureter ECM.In the first control group of 20 rabbits the resected ureter was replaced by allograft transplantation of untreated ureter.In the second control group of rabbits the resected ureter site was left untreated for autoregeneration.The neoureter segment was studied with electron microscopy and immunohistochemical procedures. Results In the experimental animals,transitional epithelial cells and monocytes could be seen in the ECM area and fully filled up the area after 3 weeks.No stenosis and hydronephrosis were observed on IVU 24 weeks after replacement of the resected ureter.The histological structure was not much different from a normal ureter.In the first control group with allograft transplantation,rejection and necrosis occurred 7~10 days after the transplantation.In the second conrol group of autoregeneration the studied area was embeded with fibrosis tissue.On the basis of the experiment,ureter ECM has been successfully used in clinical practice. Conclusions Alloureter ECM might be an ideal replacement material for ureter.

【关键词】 输尿管细胞外基质移植
【Key words】 Ureter Extracellular matrix Transplantation
  • 【文献出处】 中华泌尿外科杂志 ,CHINESE JOURNAL OF UROLOGY , 编辑部邮箱 ,1999年10期
  • 【分类号】R318.16
  • 【被引频次】24
  • 【下载频次】87
节点文献中: 

本文链接的文献网络图示:

本文的引文网络