【摘要】 目的　比较不同厂家生产的酸化电位水生成机制备的酸化电位水对微生物的杀灭效果，并对杀菌因子和影响因素进行探讨。方法　应用悬液定量杀灭试验检测酸化电位水对金黄色葡萄球菌、大肠杆菌和枯草杆菌黑色变种芽胞的杀灭效果，ＨＣｌ＋ 次氯酸钠和蒸馏水＋ 次氯酸钠对金黄色葡萄球菌的杀灭效果及有机物对酸化电位水杀灭枯草杆菌黑色变种芽胞效果的影响。应用ＨＢｓＡｇ 抗原性悬液定性破坏试验检测酸化电位水对ＨＢｓＡｇ 抗原性的破坏效果。结果　所试厂家的生成机制备的酸化电位水对金黄色葡萄球菌、大肠杆菌作用３０ ｓ 其平均杀灭率均为１００ ％ ，对ＨＢｓＡｇ 作用３０ｓ，可破坏ＨＢｓＡｇ 的抗原性。蒸馏水中加入次氯酸钠，其杀菌效果与酸化电位水相同。在枯草杆菌黑色变种芽胞悬液中加与不加１％ 的蛋白胨， 酸化电位水对该芽胞作用２０ｍｉｎ ，其平均杀灭率分别为５９ ．５４ ％ 和１００ ％ 。结论　所试厂家的生成机制备的酸化电位水对金黄色葡萄球菌、大肠杆菌和ＨＢｓＡｇ 具有快速的杀灭和破坏作用。酸化电位水的主要杀菌因子是有效氯。１ ％ 蛋白胨对酸化电位水杀灭芽胞的效果有明显影响更多还原

【Abstract】 Objective To compare the germicidal efficacy of Electrolyzed Oxidizing Water (EOW) from instruments offered by the manufactures.To approach bactericidal factors of EOW and factors influencing the germicidal efficacy. Methods A quantitative suspension test was used for detecting the germicidal efficacy of EOW against Staphy aureus, Escherichia coli and spores of Bacillus subtilis var.niger. This test was also used for detecting the germicidal efficacy of HCl+NaOCl against Starphy aureus and observing the influence of 1% peptone on efficacy of EOW in killing spores of Bacillus subtilis var.niger. Results It was found that EOW produced by every instrument of the manufactures with 30 seconds contact-time could attain a killing rate of 100% for Starphy aureus and E.coli and could destroy the antigenicity of HBsAg. The germicidal efficacy of the NaOCl dissolved in distilled water against Starphy aureus was the same as of EOW. As the suspension of spores of Bacillus subtilis var. niger containing or no containing 1% peptone, EOW with 20 minutes contact time could attain a killing rate of 59.94% or 100% respectively. Conclusion EOW produced by instruments of all the manufactures can rapidly kill or destory the Starphy aureus , Escheichia coli , the antigenicity HBsAg. The main germicidal factor of EOW is available chlorine. The 1% peptone has significant influence on germicidal efficacy of EOW.更多还原