【摘要】 目的　短小芽胞杆菌Ｅ６０１ 已在中国确定为辐射灭菌指示菌。为使指示菌在灭菌过程中的应用标准化、规范化，保证灭菌质量，需要对其固有抗辐射性和影响因素，以及如何制备抗辐射稳定的生物指示剂作详尽研究。方法　应用钴６０γ射线和高能电子束对短小芽胞杆菌Ｅ６０１ 进行辐照，测定不同条件下的Ｄ１０ 值。结果　①因载体、介质不同，Ｄ１０ 值变化在１ ．６０ ～２．５３ｋＧｙ 之间。②不同温度下保存一年，指示菌片芽胞抗辐射性无明显变化。但随着保存时间延长，菌片上存活菌数逐渐减少。③载体、介质影响存活率，电子束与钴６０ 结果一致。结论　短小芽胞杆菌Ｄ１０ 值在１ ．６０～２．５３ｋＧｙ 之间，且抗性稳定。本试验条件下制备的可溶性菌片抗力高，Ｄ１０ 值为２ ．５３ｋＧｙ，可长期保存，适宜作指示剂应用更多还原

【Abstract】 Objective In order to confirm the radiation resistance and stability of Bacillus pumilus E 601 and to further standardize and normalize the application of this bacterium as biological indicator in the practice of radiation sterilization. Methods B.pumilus ,suspended at different solutors and at different mediums was radiated 4 times at interval of 3 months for one year by Co-60 r-ray. According to the number of survival bacteria cells, D 10 values were calculated. Results ①The D 10 values are varied from 1.60- 2.53kGy as the results from different mediums and carriers. ②Under the storage at different temperature for one year,the biological indicator’s D 10 values had no significantly change but the number of living bacteria cells gradually reduced. ③The percentage of living cells was affected by the carrier and temperature. Conclusion The results of this research suggested that the radiation resistance of B.pumilus spores was relatively high. The D 10 values were in the range of 1.60- 2.53kGy and the spore innate resistance was stable,indicating that B.pumilus E 601 is a suitable indicator bacterium for ionizing radiation sterilization. The biological indicator was able to be stored for a long time without significant resistance changes. It could be prepared under the standard condition for commercial use.更多还原