【摘要】 目的　探讨老年性阳萎的发病机制。　方法　取不同月龄雄性Ｗｉｓｔａｒ大鼠阴茎海绵体组织，行特异性抗神经型一氧化氮合成酶（ｎＮＯＳ）免疫组化染色，计数ｎＮＯＳ神经纤维含量。　结果　１９～２１ 月龄组大鼠阴茎中阳性染色纤维数为（５０．８３±４．２２）条，明显少于５ 月龄组的（１２８．８８±３６．７８）条和１０ 月龄组的（１０６．２５±１２．０６）条，差异均有显著性（Ｐ＜ ０．０１），而１０ 月龄组与５月龄组差异无显著性（Ｐ＞ ０．０５）。　结论　衰老可造成大鼠阴茎中ｎＮＯＳ的减少，可能与老年性阳萎的发病有关更多还原

【Abstract】 Objective In order to elucidate the etiology of aging related erectile dysfunction,the changes of penile neural nitric oxide synthase (nNOS) content in aged rats were evaluated. Methods Eight 5 month old,eight 10 month old and six 19 21 month old male Wistar rats were studied. The nNOS content was assayed semiquantitatively in penile tissues by immunohistochemical staining with selective antibody to nNOS (1∶50 dilution). Results The number of NOS containing nerve fibers was significantly less in the 19 21 month old rats than in the 5 month and 10 month old rats 〔 50 83±4 22 vs 128 88±36 78 ( P <0 01) and 106 25±12 06( P <0 01)〕 , and those in the latter two groups were similar ( P >0 05). Conclusions Aging can cause an impairment of penile nNOS in rats; this may be associated with aging related erectile dysfunction.更多还原