【摘要】 目的建立耐长春新碱（Ｖｉｎｃｒｉｓｔｉｎｅ，ＶＣＲ）人舌鳞癌细胞系。方法采用间歇性加药，逐步提高培养基中ＶＣＲ的浓度，连续体外诱导，培养，建立了一株耐ＶＣＲ人舌鳞癌细胞系Ｔｃａ８１１３／Ｖ１００。对其药物敏感性、倍增时间、裸鼠成瘤性、超微结构及耐多药基因等生物学特性进行了研究。结果Ｔｃａ８１１３／Ｖ１００在含ＶＣＲ１００μｍｏｌ／Ｌ的培养基中稳定生长，其对ＶＣＲ的耐药性为Ｔｃａ８１１３细胞系的１９２倍，对其他几种抗癌药物也有程度不同的耐药性。Ｔｃａ８１１３／Ｖ１００倍增时间３７２小时，具有裸鼠成瘤性，病理诊断为鳞状细胞癌，超微结构观察其微绒毛短粗，ｍｄｒ１ｍＲＮＡ表达水平明显增高。结论Ｔｃａ８１１３／Ｖ１００细胞系已具备多药耐药性的特点，为口腔癌多药耐药性研究提供了细胞模型。更多还原

【Abstract】 Objective To establish a VCRresistant cell line from human squamous cell carcinoma. Methods The cell line Tca8113 was utilized. The VCR concentration was increased gradually in consecutively passage of cultures. Results A VCRresistant cell line Tca8113/V100 from Tca8113 was established, the cells grew steadily in 100 mol/L medium and with 19.2 times of resistance to VCR. The cell line was also resistant to ADM, PYM, CDDP, MTX, 5Fu at different degrees. The doubling time of the cells was 37.2 hours and showed microscopically and ultrastructurally typical squamous cell carcinoma when inoculated in nude mice. Blot hybridization showed the cells had increased espression of MDR1mRNA. Conclusion These results indicate that the cell line has multidrug resistant ability, and the reason for this may be over expression of MDR1 gene. This cell line provides an important model for the study on drugresistant cell both in cellular and molecular level, and on the mechanism of drug resistance.更多还原