【摘要】 目的阐明在肺纤维化过程中肺成纤维细胞（ＬＦ）产生透明质酸（ＨＡ）、白细胞介素８（ＩＬ８）和胶原的动态变化及作用。方法检测博莱霉素（ＢＬＭ）致大鼠肺纤维化不同时期ＬＦ培养上清中ＩＬ８活性、ＨＡ和羟脯氨酸（ＨＹＰ）水平。结果ＢＬＭ组ＬＦ的上清中（１）ＨＡ水平于第１天明显升高［（３２９±０．７０）μｇ／ｍｌ］，第３天达高峰［（６．１２±１．２３）μｇ／ｍｌ］，随后降低，第１４～２８天时降至正常；（２）ＩＬ８活性于第１天明显增高，每高倍镜视野细胞数为４０６±０７９，第７天达高峰，每高倍镜视野细胞数为９３６±１１０，随后降低，第２８天降到正常；（３）胶原含量于第７天明显增多［（２．４４±０．３２）μｇ／ｍｌ］，第１４天达峰值［（２．８０±０．３６）μｇ／ｍｌ］，以后降低，但至第２８天仍高于正常［（１．９６±０．４４）μｇ／ｍｌ］；（４）ＨＡ和ＩＬ８水平与支气管肺泡灌洗液（ＢＡＬＦ）细胞成分之间均具有明显的正相关。结论在肺纤维化过程中ＬＦ在早期肺泡炎阶段就处于活化状态，产生ＨＡ和ＩＬ８增多，是肺内ＨＡ和ＩＬ８的主要来源之一；ＬＦ产生ＨＡ和ＩＬ８增多可反映肺泡炎的强度和疾病的活动性；肺内胶原的过多?更多还原

【Abstract】 Objectives The purpose of the present study was to clarify the consecutive changes and roles of hyaluronan (HA), interleukin 8 (IL 8) and collagen production by lung fibroblasts in the course of pulmonary fibrosis. Methods Quantitative and comparative assessments of the HA,IL 8 and collagen (presented by hydroproline;HYP) levels in lung fibroblast conditioned media were made at various stages during the development of bleomycin induced pulmonary fibrosis in rats. Results In lung fibroblast conditioned media of bleomycin treated animals: (1) The HA levels increased significantly on day 1,peaked on day 3, and then gradually declined and returned to control values on days 14～28. (2) The IL 8 levels strikingly increased on day 1,reached the peak values on day 7,thereafter,gradually decreased,regained control values by day 28. (3) The collagen levels increased significantly on day 7,peaked on day 14,and then gradually declined, However,remained significantly above normal values on day 28. (4) In bleomycin group,the HA and IL 8 levels both significantly correlated to cell components in BALF. Conclusions The lung fibroblasts were activated,produced increased HA and IL 8,and were the main source of HA and IL 8 within the lung in the early stage of pulmonary fibrosis; increased HA and IL 8 synthesis of lung fibroblasts might reflect the intensity of alveolitis and the disease activity;The excessive collagen deposition within the lung primarily occurred at the middle stage of pulmonary fibrosis.更多还原