【摘要】 目的探讨内毒素在体外直接作用于大鼠肝细胞时，肝细胞所发生的病理及生物化学特性的变化。方法采用胶原酶灌注法分离肝细胞，体外培养后在培养基中直接加入内毒素，用光镜和透射电镜观察其形态学变化Ｚ提取肝细胞的Ｄ＼Ａ片段经琼脂糖凝胶电泳观察肝细胞生物化学性质的改变。结果内毒素直接作用的肝细胞在形态学上表现出染色质凝集等凋亡细胞的特征；在生物化学方面表现典型的凋亡梯形，此过程可以被凋亡抑制剂金红三羧酸（ａｕｒｌｎｔｒｉｃａｒｂｏｘｙｌｉｃ　ａｃｉｄ，ＡＴＡ％制。肝细胞调亡的数量随内毒素剂量的增加而增加，并且在处理前２４小时之内随处理时间的延长而增加。结论内毒素在体外直接作用于肝细胞，可诱导肝细胞发生凋亡。更多还原

【Abstract】 Objectives To investigste the effect of lipopolysaccharide(LPS) on hepatocyte in vitro.Medthods The hepatocytes were isolated in the way of liver per fusion with 0.05% collagrnase type Ⅰ andtype Ⅳ, and cultured for 24h in vitro before LPS was added directly into the culturing medium.Propidium iodide(PI) staining, and transmission electron microscopy techniques had been used to obsservethe morphological changes of hepatocyte treated with LPS. DNA-fragment assay was analyzed by theagurose gel electrophoresis to determine apoptostic level. Results Hepatocytes incubated with LPS exhib-ited some typical apoptosis-speific morptiological features. The DNA-fragment by agsrose gel electro- phoresis demonstrated the typical ladder pattern on the hepatocytes directly exposed to LPS, but it wasabsent in the group used ATA, an inhibitor of apoptosis. These morphological chances, accompanied byDNA fragmentation assay, confirmed that cells were dying through an apoptotic pathway. In addition, thehepatocyte number of apoptosis increased parallel with the dose of LPS and time within 24h when hepa- tocytes were exposed to LPS alone. Conclusions LPS can induce apoptosis of hepatocyte in vitro.更多还原