【摘要】 研究甘氨脱氧胆酸（ＧＤＣＡ）对肝细胞脂质过氧化及细胞膜流动性的影响，探讨ＧＤＣＡ肝损伤机理。方法以ＧＤＣＡ为处理因素，体外培养肝细胞。结果终浓度为２５０μｍｏｌ／Ｌ时，ＧＤＣＡ组培养１～４小时，肝细胞ＭＤＡ含量显著增加（Ｐ＜０００１），细胞膜流动性显著降低（Ｐ＜０．０２）。随着丙二醛（ＭＤＡ）的增加和细胞膜流动性的降低，肝细胞释放ＡＬＴ增加，ｒ分别是０．９４５和０９８６。结论ＧＤＣＡ能引起肝细胞ＭＤＡ增加和膜流动性降低，从而导致肝细胞损伤，从新的角度解释了ＧＤＣＡ的肝损伤机理。更多还原

【Abstract】 Objecdtives To explore the mechanisms of hepatocyte injury caused by glycodeoxycholicacid(GDCA) through studies on the roles of GDCA to membrane fluidity and superoxides of lipids ofhepatocyte. Methods GDCA was used to treat hepatocytes of rat cultured in vitro and changes in cellmembrane fluidity and malondialdehyde(MDA) were observed. Results in the presence of GDCA(finalconcentration of 25mol/L), the concentration of hepatocyte MDA of GDCA increase significantiy (P<0.001)and membrane fluidity decreased(P<0.02) after culture of I or 4 hours. ALT was directly realted to MDA andfluorescence polarization(P), r was 0.945 and 0.986, respectively Conclusion GDCA can induce the increase ofMDA and decrease of fluidity of hepatocyte membrane, and finally cause hepatocyte injury.更多还原