【摘要】 目的　研究受１５３ＳｍＥＤＴＭＰ内照射治疗时诱发骨肉瘤细胞凋亡的ＤＮＡ损伤特征，以及１５３Ｓｍ 核素在瘤细胞中的行径特性。方法　观察了１５３ＳｍＥＤＴＭＰ内照射不同时间，诱发骨肉瘤细胞凋亡的ＤＮＡ 凝胶电泳和微观放射自显影的示踪动态。结果　研究发现，在１５３ＳｍＥＤＴＭＰ内照射作用下，骨肉瘤细胞的ＤＮＡ 呈现出在核小体间断裂的阶梯状条带形成的凋亡特征，放射性核素１５３Ｓｍ 可迅速透过瘤细胞膜，也可被瘤细胞吞噬，呈亲膜性积聚和膜包裹着的凋亡小体呈现。结论　１５３ＳｍＥＤＴＭＰ内照射治疗时，可诱发骨肉瘤细胞凋亡发生，其诱发凋亡的程度随１５３Ｓｍ 内照射作用时间延长而增升。更多还原

【Abstract】 Objective\ To study the characteristics of injury in apoptotic bone tumor cells and behavior of radionuclide \{\}\+\{153\}Sm in tumor cells. Methods DNA gel electrophoresis and microautoradiographic tracing of apoptotic bone tumor cells at different intervals after \{\}\+\{153\}Sm\|EDTMP internal irradiation. Results Bone tumor cells internally irradiated with 153 Sm\|EDTMP displayed characteristics of DNA ladder formation in apoptotic cells. 153 Sm could permeate through tumor cell membrane and be phagocytized by the tumor cells,showing membrane\|seeking and membrane bounded apoptotic bodies condensation. Conclusion Progression of apoptosis in bone tumor cells induced by 153 Sm\|EDTMP is dependent on the time elapse of 153 Sm internal irradiation.\;更多还原