【摘要】 目的探讨Ｉ型膜型基质金属蛋白酶（ＭＴ１ＭＭＰ）在人肿瘤细胞系中的表达情况及其与明胶酶Ａ（ＭＭＰ２）的分泌和活化的关系。方法运用逆转录巢式ＰＣＲ、ＲＮＡ印迹分析和蛋白质印迹分析的方法，检测ＭＴ１ＭＭＰｍＲＮＡ和ＭＭＰ２蛋白在４个黑色素瘤、２个肺癌和２个前列腺癌细胞系中的表达水平。结果ＭＴ１ＭＭＰｍＲＮＡ在所有癌细胞系中均有表达，但表达水平很不一致：在４个黑色素瘤细胞系中的表达水平明显高于肺癌和前列腺癌细胞系；仅在黑色素瘤细胞系的无血清条件培养液中，检测到了活化型ＭＭＰ２，而人肺癌细胞系ＰＧ虽然分泌酶原型ＭＭＰ２，但不产生活化型ＭＭＰ２。结论ＭＴ１ＭＭＰｍＲＮＡ的表达具有一定的组织特异性，并与ＭＭＰ２的活化密切相关。更多还原

【Abstract】 Objective To study the expression of membranetype I matrix metalloproteinase (MT1MMP) and its correlation with gelatinase A (MMP2) activation. Methods Reverse transcription PCR, northern blots and western blots were employed to detect the expression levels of MT1MMP mRNA and MMP2 protein in 4 human melanoma cell lines, 2 human lung cancer cell lines and 2 human prostate cancer cell lines. Results All the cancer cell lines expressed MT1MMP mRNA, and the MT1MMP mRNA level in the 4 melanoma cell lines was significantly higher than that in the lung and prostate cancer cell lines. Activated MMP2 proteins were only detected in the melanoma cell lines, whereas PG, a lung cancer cell line, which secreted proMMP2 and expressed low level of MT1MMP, could not produce activated MMP2. Conclusions The level of MT1MMP expression was highly associated with MMP2 activation.更多还原