【摘要】 为了探讨白血病细胞的分化和端粒酶活性改变的关系。方法：采用ＰＣＲ－ＥＬＩＳＡ方法检测白血病细胞株Ｋ－５６２和ＨＬ－６０诱导分化前后的端粒酶活性。结果：未经药物处理的Ｋ－５６２和ＨＬ－６０细胞有高的端粒酶活性；用苦参碱作用Ｋ－５６２细胞和维甲酸处理ＨＬ－６０细胞一定时间后，端粒酶活性被明显抑制：分化诱导剂不能直接抑制端粒酶活性；端粒酶活性的高低与细胞所处的分化状态有关。结论：分化是细胞自身调控的过程，端粒酶活性的高低与细胞所处的分化状态密切相关。更多还原

【Abstract】 Purpose: To study the relationship between differentiation of leukemia cells andalteration of telomerase activity. Methods: Telomerase activity was evaluated by PCR - ELISA duringand following induced differentiation in K - 562 and HL -60 cells. Results: Untreated K -562 andHL - 60 cells had higher telomerase activity which was significantly inhibited in K - 562 cells treated withMatrine and in HL - 60 cells treated with retinoic acid. The differentiation - inducing agents which hadnot directly inhibit telomerase activity indicated that the inhibition of telomerase activity responded to theinduction of differentiation and the loss of telomerase activity was closely associated with the state of thecellular differentiation. Conclusion: Cellular differentiation is a self - regulation process of the cells and,telomerase activity is associated with the state of cell differentiation.更多还原