【摘要】 目的　为基因治疗寻找有效的基因转移方法。方法　以腺病毒为载体，以聚阳离子化合物介导和直接应用裸质粒这三种基因转移方法，观察了转染以后神经胶质母细胞瘤细胞对ＧＣＶ药物的敏感性。结果　应用ＧＣＶ ７２小时后，腺病毒载体组细胞生存率仅为２３％ ，而聚阳离子化合物介导的基因转移组和直接应用裸质粒组的细胞生存率分别为５７％ 和９６％ ，远远高于腺病毒载体组。结论　腺病毒介导的基因转移方法是胶质瘤基因治疗的有效手段。更多还原

【Abstract】 ? Objective To find effective gene transfer methods for gene therapy of glioma.Methods The plasmid pACCMV TK which expresses herpes simple virus thymidine kinase(HSV TK)gene was constructed,and recombinant adenovirus AdCMV TK was gained.Glioma cell TJ905 was treated with AdCMV TK,polybrene/pACCMV TK and plasmid pACCMV TK respectively and their GCV sensitivity was observed.Results Proliferation of TJ905 cells infected with AdCMV TK was inhibited evidently.The cell survival rate was 23% in the third day after GCV was used,which was remarkably lower than those of the cells treated with polybrene/pACCMV TK(57%) and plasmid pACCMV TK(96%).Conclusion Recombinant AdCMV TK is a feasible gene therapeutic tool for glioma. 〔更多还原