【摘要】 目的 :观察丁基苯酞 ( 3 n butylphthalide ,NBP)对低糖低氧刺激后培养的胎鼠皮层神经细胞谷氨酸和 5 HT释放的影响。方法 :胎鼠皮层神经元以低糖低氧培养基培养造成低糖低氧模型 ;谷氨酸和 5 HT以荧光分光光度法进行测定。结果 :低糖低氧 1 0h可使神经细胞谷氨酸的释放量增加 ,dl NBP( 1和 1 0 μmol·L-1)和l NBP( 1 0 μmol·L-1)可降低细胞培养基中谷氨酸的浓度 ,而同剂量的d NBP对谷氨酸的释放无明显影响。dl NBP和l NBP亦可抑制由花生四烯酸 ( 1 0 0 μmol·L-1)引起的神经细胞谷氨酸的释放。本实验结果还显示 ,dl NBP( 1和 1 0 μmol·L-1)及l NBP( 1 0 μmol·L-1)可降低低糖低氧后神经细胞培养基中 5 HT的浓度。结论 :dl NBP和l NBP可抑制谷氨酸和 5 HT的释放 ,这可能是其抗脑缺血作用的机理之一。更多还原

【Abstract】 OBJECTIVE:To investigate the effects of dl 3 n butylphthalide ( dl NBP), d NBP and l NBP on the release of glutamate and 5 hydroxytryptamine (5 HT) from cultured perinatal cortical neurons.METHODS:The cortical neurons were cultured with medium containing low glucose and bubbled with N 2,Glutamate and 5 HT were determined fluorometrically.RESULTS:The results showed that dl NBP (1 and 10μmol·L -1 ) and l NBP (10μmol·L -1 ) reduced the glutamate release and the release of 5 HT from cultured cortical neurons induced by hypoglycemia/hypoxia for 10 h. d NBP was shown no significant effect. dl NBP and l NBP also decreased the glutamater elease induced by arachidonic acid (100μmol·L -1 ).CONCLUSION:The inhibitory effects of NBP on the release of glutamate and 5 HT may be one of its action mechanisms in the treatment of cerebral ischemia.更多还原