【摘要】 目的：观察不同阻断时间对体外循环期间血浆脂质过氧化物丙二醛（ＭＤＡ）和红细胞内超氧化物歧化酶（ＳＯＤ）的影响。方法：选择２９例体外循环下心内直视手术病人，根据阻断主动脉时间分为３组，第一组：阻断主动脉时间＜３０ｍｉｎ，共１２例；第二组：阻断主动脉时间３０～６０ｍｉｎ，共１０例：第三组：阻断主动脉时间＞６０ｍｉｎ，共７例。分别在手术前、转流５ｍｉｎ时、开放主动脉时、停体外循环时、停体外循环后１ｈ，经桡动脉采血５ｍｌ。血清ＭＤＡ采用硫代巴比妥酸（ＴＢＡ）法测定，ＳＯＤ采用亚硝酸盐法。结果：第一组体外循环开始后，ＳＯＤ即下降，开放主动脉时ＳＯＤ明显下降，一直持续到术后（Ｐ＜０．０５）；第二组在开放主动脉时ＳＯＤ下降明显约为３０％（Ｐ＜０．０１），一直持续到术后（Ｐ＜０．０１）；第三组变化同第二组，但ＳＯＤ下降的程度更明显。第一组体外循环开始后，ＭＤＡ变化不明显（Ｐ＞０．０５）；第二组在开放主动脉时ＭＤＡ明显增加Ｐ＜０．０５），一直持续到术后１ｈ（Ｐ＜０．０１）；第三组在转流前及开放主动脉时变化同第二组，ＭＤＡ增加的程度更明显。而且随着阻断时间的延长ＭＤＡ随之增加。结论：随着阻断时间的延长，ＭＤＡ随之增加，ＳＯＤ随?更多还原

【Abstract】 Objective:To investigate the effects of ischemia time on plasma malondialdehyde (MDA) and superoxide dismutase(SOD) during cardiopulmonary bypass (CPB).Methods:Twenty nine patients undergoing open heart surgery were assigned into three groups:group I (n=12) inchemia time<30min;group Ⅱ(n=10) ischemia time 30～60 min;group Ⅲ(n=7) ischemia time>60 min.Plasma MDA were measured by thiobarbituric acid (TBA) method, SOD were measured by nitrite method. [WT5”HZ〗Results:[WT5”BZ〗Plasma MDA increased gradually before reperfusion and increased significantly after reperfusion in group I. In group Ⅱ plasma MDA gradually and slightly increased. At l hour after CPB in group Ⅱ plasma MDA increased remarkably as compared with group I(P<0.01). In both group, SOD decreased significantly before and after reperfusion (P<0.01), but SOD recovered gradually after reperfusion (P<0.05) in group Ⅱ.Conclusion:Changes of plasma MDA and SOD were related to the duration of myocardium ischemia.更多还原