【摘要】 目的：观察手术和体外循环转流前后血浆白介素－８的含量变化。方法：选择１７例体外循环下心内直视手术病人，分别在手术前、转流５ｍｉｎ时、开放主动脉时、停体外循环后１ｈ，经桡动脉采血２ｍｌ，用酶联免疫法测定白介素－８。结果：体外循环开始时白介素－８有上升趋势（Ｐ＞０．０５），开放主动脉后白介素－８增加明显，由（３１．７５±５．０５）ｐｇ／ｍｌ增加到（９３．７１±３０．７４）ｐｇ／ｍｌ（Ｐ＜０．０５），停体外循环后白介素－８仍持续增加为（２８５．８４±１８６．８６）ｐｇ／ｍｌ（Ｐ＜０．０１）。结论：体外循环期间和术后白介素－８产生增加，提示手术和体外循环可抑制病人的免疫功能更多还原

【Abstract】 Objective:To evaluate the changes of interleukin 8 during and after cardiopulmonary bypass. Methods:We measured interleukin 8 in seventeen patients with ventricular septal defect(VSD) atrial septal defect(ASD) or mitral stenoinsufficiency(MSI). Plasma interleukin 8 concentration was determined using the Human interleukin 8 Elisa Kit and a highly sensitive sandwich enzyme immunoassay. Results:Plasma interleukin 8 concentration during cardiopulmonary bypass(93.71±30.74 pg/ml) and after cardiopulmonary bypass(285.84±186.86 pg/ml) were significantly higher than that before cardiopulmonary bypass(31.75±5.05 pg/ml). Conclusion:The increase of interleukin 8 concentration during cardiopulmonary bypass demonstrated that operation and/or cardiopulmonary bypass might induce depression of immunity in these patients.更多还原