【摘要】 目的：探讨幽门螺杆菌（ Ｈ Ｐ）感染与胃癌的关系。方法： Ｐ Ｃ Ｒ 技术及免疫组化技术。结果：１３６ 例胃粘膜活检标本， 正常胃粘膜 Ｐ Ｃ Ｎ Ａ 指数为（９７８±３６５）％ ，ｐ１６ 蛋白阳性率为 １００％ ；胃良性病变 Ｈ Ｐ阴性组 Ｐ Ｃ Ｎ Ａ 指数为（１３９４±７３４）％ ，ｐ１６ 蛋白阳性率为９５３％ ；胃良性病变 Ｈ Ｐ阳性组 Ｐ Ｃ Ｎ Ａ 指数为（２０７１±１１２６）％ ，ｐ１６ 蛋白阳性率为 ８２１％ 。 Ｈ Ｐ阳性组与 Ｈ Ｐ阴性组比较， Ｐ Ｃ Ｎ Ａ 指数两者之间差异有非常显著意义（ Ｐ ＜ ００１），ｐ１６ 蛋白阳性率两者之间差异有显著意义（ Ｐ＜ ００５）。结论： Ｈ Ｐ阳性组 Ｐ Ｃ Ｎ Ａ 指数增加，ｐ１６ 蛋白表达减弱， Ｈ Ｐ感染可能抑制了抑癌基因ｐ１６ 的活性，刺激了胃粘膜细胞的过度增殖， Ｈ Ｐ感染是胃癌发生的重要危险因素。更多还原

【Abstract】 Objective:This paper was to explore the relationship between helicobacter pylori(HP) infection and gastric carcinoma.Methods:We detected the HP by polymerase chain reaction(PCR) and studied the(PCNA)and p16 expression by immunohistochemistry for 136 specimens.Results: The PCNA index(PI) of normal gastric mucosa. HP negative gastric benign lesions, HP positive gastric benign lesions was (9.78±3.65)%,(13.94± 7.34 )%,and (20.71±11.26)%,respectively. The PI showed significant difference between HP positive benign lesions and HP negative benign lesions(P<0.01). The positive rate of p16 protein expression was 100%,95.3% and 82.1% for normal gastric mucosa,HP negative and HP positive benign lesions,respectively.There was a statistical difference between the HP negative and HP positive benign lesions.Methods:We detected the HP by polymerase chain reaction(PCR) and studied the(PCNA)and p16 expression by immunohistochemistry for 136 specimens.Results: The PCNA index(PI) of normal gastric mucosa. HP negative gastric benign lesions, HP positive gastric benign lesions was (9.78±3.65)%,(13.94± 7.34 )%,and (20.71±11.26)%,respectively. The PI showed significant difference between HP positive benign lesions and HP negative benign lesions(P<0.01). The positive rate of p16 protein expression was 100%,95.3% and 82.1% for normal gastric mucosa,HP negative and HP positive benign lesions,respectively.There was a statistical difference between the HP negative and HP positive benign lesions.We detected the HP by polymerase chain reaction(PCR) and studied the(PCNA)and p16 expression by immunohistochemistry for 136 specimens.Results: The PCNA index(PI) of normal gastric mucosa. HP negative gastric benign lesions, HP positive gastric benign lesions was (9.78±3.65)%,(13.94± 7.34 )%,and (20.71±11.26)%,respectively. The PI showed significant difference between HP positive benign lesions and HP negative benign lesions(P<0.01). The positive rate of p16 protein expression was 100%,95.3% and 82.1% for normal gastric mucosa,HP negative and HP positive benign lesions,respectively.There was a statistical difference between the HP negative and HP positive benign lesions.Results: The PCNA index(PI) of normal gastric mucosa. HP negative gastric benign lesions, HP positive gastric benign lesions was (9.78±3.65)%,(13.94± 7.34 )%,and (20.71±11.26)%,respectively. The PI showed significant difference between HP positive benign lesions and HP negative benign lesions(P<0.01). The positive rate of p16 protein expression was 100%,95.3% and 82.1% for normal gastric mucosa,HP negative and HP positive benign lesions,respectively.There was a statistical difference between the HP negative and HP positive benign lesions.Conclusion:HP infection might cause the overproliferation of gastric epithelial cell by inhibiting the activity of p16 suppressor gene and might enhance the susceptibility of canceration for gastric epithelial cell.更多还原