【摘要】 采用卡拉胶固定柑桔黄单胞菌 X0 34进行头孢氨苄的生产 ,控制 p H6.2左右 ,2 5℃ ,底物 7- ADCA的浓度为 2 .0 % ,侧链与底物配比 ( PGME/7- ADCA)为 3.0时可得到较高的转化率。用戊二醛硬化 ,可连续反应32次。细胞载量 [菌体重量 ( g) /固定化介质重量 ( g) ]控制在 15%左右 ,可使固定化细胞有较好的机械强度及较高的酶活性更多还原

【Abstract】 For continuously producing of cephalexin, whole cells of \%Xanthomonas citri\% were immobilized by entrapment in kappa carrageenan gel. The immobilized cells showed maximal conversion at pH 6.2. The concentration of 7 ADCA was 1.5%～2.0% and PGME/7 ADCA was about 3.0, in which the reaction could take place completely. Those cells with hardening by glutaraldehyde could continue to product 32 times. The load of enzyme was kept at 15%, and it showed high enzyme activity and strength.更多还原