【摘要】 用 Fluo 3- AM为荧光探针 ,测定 2型糖尿病患者淋巴细胞内 Ca2 +浓度 ,观察了肾上腺素、多巴酚丁胺、Bay K 864 4、胰岛素等药物对胞内 Ca2 +的作用机制。实验表明患者胞浆内 Ca2 +浓度与正常人相比显著升高 ;药物对胞内 Ca2 +的刺激表明 ,病人对外界钙激动剂更敏感。胰岛素能刺激胞内 Ca2 +浓度升高 ,其作用机制是激活钙离子通道。肾上腺素不仅能激活钙离子通道 ,也能促使钙从钙池中释放。上述研究也表明 Fluo- 3是有效的研究胞内 Ca2 +变化的荧光探针。 2型糖尿病的发生可能与 Ca2 +浓度升高有关 ,患者对外界钙激动剂更敏感更多还原

【Abstract】 The purpose of the research was to determine the intracellular calcium of patients of NIDDM. We also analyzed the mechanism of several drugs such as Adrenaline, Dobutamine, Bay K 8644 and Insulin which stimulated calcium release of NIDDM, using Fluo 3 AM as fluorescent probe. The results showed that the concentration of intracellular calcium increased compared with healthy persons. Patients are more sensitive to Ca 2+ activator than healthy people. Insulin can stimulate calcium release which mechanism is to excite the Ca 2+ channel. Adrenaline can not only excite the Ca 2+ channel, but also stimulate the calcium release from Ca 2+ pools. The part of Ca 2+ in insulin signal transduction of NIDDM may have defects.更多还原