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诱导物对二氢嘧啶酶的作用
The Effect of Inducer on Enzyme Activity of Dihydropyri midinase
【摘要】 D对羟基苯甘氨酸可由二氢嘧啶酶水解DL对羟基苯海因制备得到。本文对Pseudomonas putida9801 菌所产生的二氢嘧啶酶诱导物进行了筛选。得出最佳的诱导物为甲硫乙基海因,最适诱导用量为0 .1 % 。与不加诱导物相比,采用分步诱导可以使酶活力增高近30 倍。
【Abstract】 The effect of several inducers on the activity of dihydropyrimidinase in grown \%Pseudomonas putida\% 9801 which biosynthesis \%D p\% hydroxyphenylglycine was studied. The best inducer is methylthioethylhydantoin. The optimum concentration of methylthioethylhydantoin is 0.1%. Compared enzyme activity of stepwise addition with enzyme activity without adding inducer, the activity of dihydropyrimidinase increases almost 30 times.
【关键词】 诱导物;
二氢嘧啶酶;
D-对羟基苯甘氨酸;
【Key words】 Inducer; Dihydropyrimidinase; \%D p\% hydroxyphenylglycine;
【Key words】 Inducer; Dihydropyrimidinase; \%D p\% hydroxyphenylglycine;
- 【文献出处】 中国药科大学学报 ,JOURNAL OF CHINA PHARMACEUTICAL UNIVERSITY , 编辑部邮箱 ,1999年03期
- 【分类号】R914
- 【被引频次】1
- 【下载频次】67