【摘要】 用TGE及PED克隆化弱毒株以1：1配比制成TGE、PED二联弱毒疫苗，毒价为107.0-7.5TCID50／0.3ml，主动免疫与被动免疫的保护率为97.7％及98％。弱毒株毒力稳定，符合制苗标准。免疫期7个月，疫苗保存期一年，疫苗接种猪对同圈饲养的非免疫猪有低效价（16～32倍）的水平感染。6个猪场的田间试验保护率为95％-98％，对紧急预防接种的防制效果更为明显。更多还原

【Abstract】 Bi-combined living vaccine was prepared with attenuated cloning TGEV and PEDV which titers were 10-7～10-7.5 TCID50/0. 3ml according to equivalent volume. The protective rate of active and passive immunity were 97. 7% and 98% respetively.the virulence of attenuated virus is stable, and measured up to vaccine products standard. The immunity duration of the vaccine was 7 months, and the vaccine could be stored 1 year at 4℃ or room temperature, Lower antibodies titers were tested in the non-immunization swine that lived with vaccinated swine. Protective rate test of field experiment in 6 piggery was 95%～98%. It was found that the vaccine had an obvious efficacy at emergent vaccination.更多还原