【摘要】 采用蛋白酶 Ｋ 消化，酚、氯仿抽提的方法提取法氏囊匀浆和细胞培养液中的鸡传染性法氏囊病病毒（ Ｉ Ｂ Ｄ Ｖ）基因组 Ｒ Ｎ Ａ，用特异的寡核苷酸引物对其 Ｖ Ｐ２ 高变区进行反转录套式 Ｐ Ｃ Ｒ 扩增。应用该方法从一个法氏囊匀浆中即可特异地检出 Ｉ Ｂ Ｄ Ｖ Ｒ Ｎ Ａ，得到的扩增产物可用于分子流行病学的进一步分析，而从感染材料的处理到扩增结果的电泳检测，在两个工作日之内可轻松完成。本实验为法氏囊病病毒的诊断和分子流行病学的分析提供了一种快速、简便、可靠的手段。更多还原

【Abstract】 A Rapid,sensitive and specific procedure was developed in order to detect infectious bursal disease virus (IBDV) RNA directly in tissue samples.Viral RNA was extracted from the bursa of Fabricius or cell cultures by digestion with proteinase K and subsequent extraction with phenol and chloroform.Viral RNA was then amplified and identified by reverse transcription(RT) and nested PCR assay using two sets of primers flanking the VP2 variable region.In this procedure,only one bursa was needed for the detection of IBDV RNA The whole detection procedure could be completed within two workdays.更多还原