【摘要】 目的：探讨血管紧张素原基因Ｍ２３５Ｔ突变与家族性原发性高血压（ＥＨ）的关系。　　方法：应用脱氧核糖核酸（ＤＮＡ）杂交和测序检定方法，对一个典型原发性高血压大家系１００名成员［分直系亲属高血压组（ｎ＝ ４５）、直系亲属正常血压组（ｎ＝ ３８）及非直系亲属正常血压组（ｎ＝ １７）］和正常血压对照家系直系亲属２１ 名（为正常对照组）成员按＜ ３５岁和≥３５岁２个年龄层的Ｍ２３５Ｔ突变进行分析。　　结果：比较４组在＜ ３５ 岁和≥３５ 岁２个年龄层的Ｍ２３５Ｔ分布。表明各年龄层中，４组间Ｍ２３５Ｔ基因型及基因频率分布未见显著不同。　　结论：血管紧张素原基因Ｍ２３５Ｔ突变与该家族性原发性高血压没有显著关联；Ｍ２３５Ｔ突变可能不是该家族性原发性高血压的遗传易感因素。更多还原

【Abstract】 Objective:To study the association between M235T variant of angiotensinogen gene (AGT) and familial essential hypertension(EH). Methods:Molecular hybridization and DNA sequencing were performed to detect the variant in all 121 subjects.There were 100 subjects in a large EH pedigree who were devided into 3 groups:direct relatives in EH ( n =45),direct relatives in normotensives( n =38),non direct relatives in normotensives( n =17).The other 21 subjects were normal controls. Results:The comparision of genotypes and gene frequency of M235T among hypertensive group and the other three normaltesive groups stratified by age at 35 years showed no significant difference in any groups. Conclusions:These results suggest that there is no significant relationship between M235T of AGT and the familial EH.M235T variant might not be a genetic susceptible factor for this familial EH.更多还原