【摘要】 目的 :对牛心包材料进行了去污剂—酶联合脱细胞研究 ,为组织工程学方法研制生物瓣提供适合的生物材料。　　方法 :用去污剂—酶四步脱细胞方法脱除牛心包组织的细胞。　　结果 :该方法脱细胞效果良好 ,且能较好地保持胶原纤维和弹性纤维原有的排列和分布。脱细胞后牛心包材料的厚度、抗拉负荷、伸长率和热皱缩温度只有轻微的减少 ,而抗拉强度没有变化。　　结论 :去污剂—酶联合脱细胞法效果良好 ,可用于组织工程的进一步研究。更多还原

【Abstract】 Objective:To investigate a method to remove cellular components from bovine pericardial tissue,resulting a scaffold for tissue engineering of heart valve or cardiovascular patch. Methods:A detergent and enzyme extraction was practiced in this study.HE staining was performed to confirm the removal of cells and Von Gieson staining,to show the integrity of collagen and elastin.The changes in tissue shrinkage temperature,and mechanical properties were also studied. Results:The cells were removed effectively from bovine pericardial tissue,while the collagen and elastin were kept intact.The mechanical properties remained unaltered.Only the tissue shrinkage temperature dropped insignificantly. Conclusion:The research work demonstrated an effective procedure to remove cells from bovine pericardial tissue while kept its mechanical properties.The acellular matrix needs to be further evaluated biochemically and ultrastructurally.This approach may eventually lead to the engineering of tissue heart valves repopulated with patients’ own cells.更多还原