【摘要】 目的 :探讨急性心肌梗塞心肌肌浆网钙释放通道的变化。　　方法 :将兔分为急性心肌梗塞组和对照组 ,急性心肌梗塞组结扎兔冠状动脉左前降支 6小时后制成急性心肌梗塞模型 ,用氚标记的兰尼定 (3H- ryanodine)对心肌肌浆网钙释放通道进行放射配基分析。　　结果 :兔实验性急性心肌梗塞梗塞区心肌肌浆网钙释放通道总结合位点数 (Bmax值 )较对照组显著降低 (93.91± 9.6 8fmol/ mg.pro比 15 5 .74± 2 3.5 0 fmol/ mg.pro,P<0 .0 1) ;两组心肌肌浆网钙释放通道平衡解离常数 (Kd值 )无显著变化(P>0 .0 5 )。　　结论 :急性心肌梗塞时心肌肌浆网钙释放通道密度降低 ,而其亲和力则无改变。更多还原

【Abstract】 Objective:To study the change of calcium release channel in cardiac sarcoplasmic reticulum in the infarcted myocardium during acute myocardial infarction. Methods:The model of acute myocardial infarction was made by liganding the rabbit left anterior descending artery. 3H ryanodine was used as radioligand to analyze calcium release channel in cardiac sarcoplasmic reticulum. Results:①The Bmax value of ryanodine receptor in acute mycardial infarction group was decreased significantly than that of control group(93 91±9 68 fmol/mg.pro vs 155 74±23 50 fmol/mg.pro, p <0 01);②There was difference of Kd value between the two groups( p >0 05). Conclusion:The ryanodine receptor density decreased significanly in infarcted mycardium.But there is no change of the affility between the ryanodine and calcium release channel of sarcoplasmic reticulum.更多还原