【摘要】 目的和方法：本文观察中毒剂量谷氨酸对大鼠大脑皮质神经细胞内Ｃａ２＋ 含量变化的影响，并对其机制进行探讨。将１ ｍ ｍｏｌ／Ｌ谷氨酸加入大脑皮质神经细胞培养液中通过激光共聚焦扫描显微镜（ｌａｓｅｒ ｃｏｎｆｏｃａｌｓｃａｎｎｉｎｇ ｍｉｃｒｏｓｃｏｐｅ，ＬＣＳＭ） 观察细胞内Ｃａ２＋ 含量的变化。结果：①１ ｍ ｍｏｌ／Ｌ谷氨酸作用后在观察的８６ 个细胞中，７１ ％ 的细胞内游离Ｃａ２＋ 浓度发生钙振荡，发生钙振荡的波型均为尖峰型：②在抑制细胞内钙库释放后加入１ ｍ ｍｏｌ／Ｌ 谷氨酸，细胞内Ｃａ２＋ 浓度上升，但没有产生钙振荡；而抑制细胞外Ｃａ２ ＋ 内流后加入１ ｍｍｏｌ／Ｌ 谷氨酸，细胞内Ｃａ２ ＋ 发生钙振荡，但振荡幅度逐渐下降直至振荡消失。结论：中毒剂量谷氨酸引起神经细胞钙振荡形成的可能机制是：细胞内钙库的释放和摄取是Ｃａ２ ＋ 发生振荡的必要条件，而振荡的维持则依赖于细胞外Ｃａ２＋ 的内流更多还原

【Abstract】 Aim and methods: The purpose of present study was to observe temporal changes of intracellular Ca 2+ induced by 1 mmol/L glutamate and explore its mechanism. Temporal changes of intracellular Ca 2+ was measured by laser confocal scanning microscope. Results: ①In cerebral cortical neurons, 1 mmol/L glutamate caused Ca 2+ oscillation found in 71% neurons (86 cells in total) and wave type as spiking. ②After inhibition of the release in intracellular Ca 2+ store, [Ca 2+ ]i raised, but wasn’t calcium oscillation. On the contrary, when added 1 mmol/L glutamate following inhibition of the extracellular Ca 2+ inflow, calcium oscillation was produced in cells, but the oscillation amplitude progressively decreased until oscillation disappeared. Conclusion: The possible mechanism of Ca 2+ oscillation (in neurons induced by glutamate of toxic dose) is that the release and uptake of Ca 2+ store are involved in Ca 2+ oscillation and Ca 2+ oscillation is dependent on the Ca 2+ influx from extracellular space.更多还原