【摘要】 以ｐＢＩ１２１质粒作外源ＤＮＡ，去壳水稻种子为浸泡受体。对Ｇ４１８抗性苗进行Ｓｏｕｔｈｅｒｎｂｌｏｔ分析，证实ｐＢＩ１２１上的ＮＰＴ正基因整合到受体基因组中。以溶解ＰＢＩ１２１的ＴＥ溶液浸泡处理为对照，用扫描电镜观察胚芽生长点外层细胞表面，结果显示，对照与ＤＮＡ溶液浸泡组两者间细胞表面结构有相似性，但ＤＮＡ溶液浸泡组出现对照组所没有的表面孔洞结构，说明ＤＮＡ可诱导生长点细胞表面产生新的结构。更多还原

【Abstract】 Rice uncoated seeds were soaked in pBI121 plasmid solution, and screened in G418 solution. The rice seedlings resistant to G418 were got. Southern blot analysis indicates that NPT Ⅱ gene in pBI121 integrated into the genome of rice acceptor. Bymeans of scanning electronic microscope, the surface of cell in apical point show that there were different microstructures betweenmaterials soaked in DNA solution and controls, which means that special gene expression must have been induced by DNAmolecules.更多还原